Kawabata Kenji, Inamura Mitsuru, Mizuguchi Hiroyuki
Laboratory of Stem Cell Regulation, National Institute of Biomedical Innovation, Osaka, Japan.
Methods Mol Biol. 2012;826:115-24. doi: 10.1007/978-1-61779-468-1_10.
Establishment of protocols for the differentiation of hepatic cells from human embryonic stem (ES) and induced pluripotent stem (iPS) cells could contribute to regenerative cell therapies or drug discovery and development. However, the differentiation efficiency of endoderm-derived cells, such as hepatic cells, from human ES and iPS cells is poor because hepatic cells are differentiated via multiple lineages including endodermal cells, hepatic progenitor cells, and mature hepatocytes. We show here the protocols for efficient hepatic differentiation from human ES and iPS cells by adenovirus vector-mediated gene transfer.
建立从人胚胎干细胞（ES）和诱导多能干细胞（iPS）分化肝细胞的方案，可能有助于再生细胞疗法或药物发现与开发。然而，从人ES细胞和iPS细胞分化内胚层来源的细胞（如肝细胞）的效率较低，因为肝细胞是通过包括内胚层细胞、肝祖细胞和成熟肝细胞在内的多个谱系进行分化的。我们在此展示了通过腺病毒载体介导的基因转移从人ES细胞和iPS细胞高效分化肝细胞的方案。
