哺乳动物细胞中的同位素标记
Isotope labeling in mammalian cells.
作者信息
Dutta Arpana, Saxena Krishna, Schwalbe Harald, Klein-Seetharaman Judith
机构信息
Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
出版信息
Methods Mol Biol. 2012;831:55-69. doi: 10.1007/978-1-61779-480-3_4.
Abstract
Isotope labeling of proteins represents an important and often required tool for the application of nuclear magnetic resonance (NMR) spectroscopy to investigate the structure and dynamics of proteins. Mammalian expression systems have conventionally been considered to be too weak and inefficient for protein expression. However, recent advances have significantly improved the expression levels of these systems. Here, we provide an overview of some of the recent developments in expression strategies for mammalian expression systems in view of NMR investigations.
摘要
蛋白质的同位素标记是将核磁共振（NMR）光谱应用于研究蛋白质结构和动力学的一种重要且常需的工具。传统上，哺乳动物表达系统被认为在蛋白质表达方面过于薄弱和低效。然而，最近的进展显著提高了这些系统的表达水平。在此，鉴于NMR研究，我们概述了哺乳动物表达系统表达策略的一些最新进展。
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