蛋白质与RNA相互作用的核磁共振研究。

NMR studies of protein-RNA interactions.

作者信息

Theimer Carla A, Smith Nakesha L, Khanna May

机构信息

Department of Chemistry, University at Albany SUNY, Albany, NY, USA.

出版信息

Methods Mol Biol. 2012;831:197-218. doi: 10.1007/978-1-61779-480-3_12.

DOI:10.1007/978-1-61779-480-3_12

PMID:22167676

Abstract

This chapter describes the preparation of NMR quantities of RNA purified to single-nucleotide resolution for protein-RNA interaction studies. The protocol is easily modified to make nucleotide-specific isotopically labeled RNAs or uniformly labeled RNA fragments for ligation to generate segmentally labeled RNAs.

摘要

本章描述了为蛋白质-RNA相互作用研究而纯化至单核苷酸分辨率的核磁共振级RNA的制备方法。该方案易于修改，以制备核苷酸特异性同位素标记的RNA或均匀标记的RNA片段用于连接，从而生成片段标记的RNA。