适用于详细核磁共振研究的蛋白质 - DNA 复合物的制备与优化。
Preparation and optimization of protein-DNA complexes suitable for detailed NMR studies.
作者信息
Sam My D, Clubb Robert T
机构信息
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.
出版信息
Methods Mol Biol. 2012;831:219-32. doi: 10.1007/978-1-61779-480-3_13.
Abstract
This chapter describes the methods to form and optimize samples of protein-DNA complexes that are suitable for detailed structure and dynamics studies by NMR spectroscopy.
摘要
本章介绍了形成和优化蛋白质 - DNA 复合物样品的方法，这些样品适用于通过核磁共振光谱进行详细的结构和动力学研究。
相似文献
1
Preparation and optimization of protein-DNA complexes suitable for detailed NMR studies.适用于详细核磁共振研究的蛋白质 - DNA 复合物的制备与优化。
Methods Mol Biol. 2012;831:219-32. doi: 10.1007/978-1-61779-480-3_13.
2
NMR studies on protein-nucleic acid interaction.蛋白质-核酸相互作用的核磁共振研究。
J Biomol NMR. 2013 May;56(1):1-2. doi: 10.1007/s10858-013-9736-8.
3
[Intermediates in macromolecular binding process as revealed by new NMR methods].[通过新的核磁共振方法揭示的大分子结合过程中的中间体]
Tanpakushitsu Kakusan Koso. 2007 Aug;52(9):952-8.
4
NMR studies of protein-RNA interactions.蛋白质与RNA相互作用的核磁共振研究。
Methods Mol Biol. 2012;831:197-218. doi: 10.1007/978-1-61779-480-3_12.
5
Macromolecular crowding in biological systems: hydrodynamics and NMR methods.生物系统中的大分子拥挤现象：流体动力学和核磁共振方法。
J Mol Recognit. 2004 Sep-Oct;17(5):397-407. doi: 10.1002/jmr.694.
6
Editorial overview: Macromolecular assemblies.
Curr Opin Struct Biol. 2020 Apr;61:vi-viii. doi: 10.1016/j.sbi.2020.02.002. Epub 2020 Mar 9.
7
Combining NMR and small angle X-ray scattering for the study of biomolecular structure and dynamics.结合核磁共振和小角X射线散射用于生物分子结构与动力学研究。
Arch Biochem Biophys. 2017 Aug 15;628:33-41. doi: 10.1016/j.abb.2017.05.005. Epub 2017 May 10.
8
TROSY-based NMR experiments for the study of macromolecular dynamics and hydrogen bonding.基于横向弛豫优化谱（TROSY）的核磁共振实验用于研究大分子动力学和氢键。
Methods Mol Biol. 2004;278:161-84. doi: 10.1385/1-59259-809-9:161.
9
NMR-based investigations into target DNA search processes of proteins.基于 NMR 的蛋白质靶 DNA 搜索过程研究。
Methods. 2018 Sep 15;148:57-66. doi: 10.1016/j.ymeth.2018.05.004. Epub 2018 May 10.
10
Solution NMR of large molecules and assemblies.大分子和组装体的溶液核磁共振
Biochemistry. 2007 Jan 16;46(2):331-40. doi: 10.1021/bi0621314.
本文引用的文献
1
The role of dynamic conformational ensembles in biomolecular recognition.动态构象集合在生物分子识别中的作用。
Nat Chem Biol. 2009 Nov;5(11):789-96. doi: 10.1038/nchembio.232.
2
Theory, practice, and applications of paramagnetic relaxation enhancement for the characterization of transient low-population states of biological macromolecules and their complexes.顺磁弛豫增强用于表征生物大分子及其复合物瞬态低丰度状态的理论、实践与应用。
Chem Rev. 2009 Sep;109(9):4108-39. doi: 10.1021/cr900033p.
3
NMR structure of the amino-terminal domain of the lambda integrase protein in complex with DNA: immobilization of a flexible tail facilitates beta-sheet recognition of the major groove.λ整合酶蛋白氨基末端结构域与DNA复合物的核磁共振结构：柔性尾巴的固定有助于对大沟的β折叠识别
J Mol Biol. 2009 May 15;388(4):682-90. doi: 10.1016/j.jmb.2009.03.041. Epub 2009 Mar 24.
4
Analysis and purification of synthetic nucleic acids using HPLC.使用高效液相色谱法分析和纯化合成核酸。
Curr Protoc Nucleic Acid Chem. 2001 May;Chapter 10:Unit 10.5. doi: 10.1002/0471142700.nc1005s01.
5
Mobility shift DNA-binding assay using gel electrophoresis.采用凝胶电泳的迁移率变动DNA结合分析
Curr Protoc Mol Biol. 2001 May;Chapter 12:Unit 12.2. doi: 10.1002/0471142727.mb1202s36.
6
PCR-based accurate synthesis of long DNA sequences.基于聚合酶链式反应的长DNA序列精确合成
Nat Protoc. 2006;1(2):791-7. doi: 10.1038/nprot.2006.103.
7
Structure of the cooperative Xis-DNA complex reveals a micronucleoprotein filament that regulates phage lambda intasome assembly.合作性Xis-DNA复合物的结构揭示了一种调节噬菌体λ整合酶体组装的微核蛋白丝。
Proc Natl Acad Sci U S A. 2007 Feb 13;104(7):2109-14. doi: 10.1073/pnas.0607820104. Epub 2007 Feb 7.
8
Direct observation of enhanced translocation of a homeodomain between DNA cognate sites by NMR exchange spectroscopy.通过核磁共振交换光谱法直接观察同源结构域在DNA同源位点之间增强的易位。
J Am Chem Soc. 2006 Jan 18;128(2):404-5. doi: 10.1021/ja056786o.
9
Structure and flexibility adaptation in nonspecific and specific protein-DNA complexes.非特异性和特异性蛋白质-DNA复合物中的结构与灵活性适应
Science. 2004 Jul 16;305(5682):386-9. doi: 10.1126/science.1097064.
10
Crystal structure of the excisionase-DNA complex from bacteriophage lambda.来自噬菌体λ的切除酶-DNA复合物的晶体结构。
J Mol Biol. 2004 Apr 23;338(2):229-40. doi: 10.1016/j.jmb.2004.02.053.
Zotero 插件