II - Q 限制内切酶——新型 II 类酶 - Suppr

Degtyarev S K, Rechkunova N I, Kolyhalov A A, Dedkov V S, Zhilkin P A

Institute of Biologically Active Compounds, SPA Vector, Berdsk, Novosibirsk Region, USSR.

Nucleic Acids Res. 1990 Oct 11;18(19):5807-10. doi: 10.1093/nar/18.19.5807.

Unique restriction endonucleases Bpu 10l and Bsil have been isolated from Bacillus pumilas and Bacillus sphaericus, respectively. The recognition sequences and cleavage points of these enzymes have been determinated as 5'-CC1TNAGC-3'/3'-GGANT1CG-5' for Bpu 10l and 5'-C1TCGTG-3'/3'-GAGCA1C-5' for Bsil. Restriction endonucleases Bpu 10l and Bsil represent a new class of enzymes which recognize non-palindromic nucleotide sequences and hydrolize DNA within the recognition sequence. Bpu 10l and Bsil recognition sequences may be regarded as quasipalindromic and the enzymes may be designated as type II-Q restriction endonucleases.

独特的限制性内切酶Bpu 10I和BsiI分别从短小芽孢杆菌和球形芽孢杆菌中分离得到。这些酶的识别序列和切割位点已确定，Bpu 10I为5'-CC1TNAGC-3'/3'-GGANT1CG-5'，BsiI为5'-C1TCGTG-3'/3'-GAGCA1C-5'。限制性内切酶Bpu 10I和BsiI代表了一类新的酶，它们识别非回文核苷酸序列并在识别序列内水解DNA。Bpu 10I和BsiI的识别序列可被视为准回文序列，这些酶可被指定为II-Q型限制性内切酶。