[用异常识别片段测定Bpu101限制性内切酶的底物特异性]
[Determination of the substrate specificity of Bpu101 restrictase with an unusual recognition segment].
作者信息
Degtiarev S Kh, Zhilkin P A, Prikhod'ko G G, Rechkunova N I, Repin V E
出版信息
Mol Biol (Mosk). 1989 Jul-Aug;23(4):1051-6.
PMID:2586500
Abstract
A new enzyme Bpu10I was isolated from Bacillus pumilus. This enzyme is not an isoschizomer of any known restriction endonucleases. The search of possible recognition sequences was carried out in sequences ABCNiDEF (i = 0.6) on substrate DNA lambda CI857, T7, pBR322. The recognition sequence and cleavage sites of restriction endonuclease Bpu10I have been determined as CCTNAGC. GGANTCG
摘要
从短小芽孢杆菌中分离出一种新的酶Bpu10I。这种酶不是任何已知限制性内切酶的同裂酶。在底物DNA λ CI857、T7、pBR322的序列ABCNiDEF（i = 0.6）中搜索可能的识别序列。限制性内切酶Bpu10I的识别序列和切割位点已确定为CCTNAGC。GGANTCG
Zotero 插件