Institut des Biomolécules Max Mousseron, UMR CNRS 5247, Université de Montpellier 1, Université de Montpellier 2, CNRS, Montpellier, France.
J Chromatogr A. 2012 Jan 6;1219:188-94. doi: 10.1016/j.chroma.2011.11.014. Epub 2011 Nov 12.
For many industrial applications, the combination of two different monomers in statistical or diblock copolymers enhances the properties of the corresponding polymer. However, during the polymerization reaction, homopolymers might be formed and can influence the properties for the applications. Consequently, the separation and the quantification of the homopolymers contained in copolymer samples are crucial. In addition, the charge density distribution of the statistical copolymer is an important characteristic for the applications. The purpose of this work was to study the characterization of a statistical copolymer of acrylic acid (AA) and diallyldimethyl ammonium chloride (DADMAC) by capillary electrophoresis (CE) in acidic conditions (cationic copolymers). For that purpose, a free solution electrophoretic separation was carried out according to the charge rate (chemical composition) independently of the molar mass. The second objective was to compare contactless conductivity detection and indirect UV absorbance modes for the quantification of DADMAC homopolymers present in copolymer samples. Different coated capillaries based on neutral or positively charged modification were also compared. The comparison of indirect absorbance UV and contactless conductimetric detection demonstrated that both detection modes can be used for a complete CE characterization of non-UV absorbing PAA-DADMAC copolymers.
对于许多工业应用来说，将两种不同单体组合成统计或嵌段共聚物可以增强相应聚合物的性能。然而，在聚合反应过程中，可能会形成均聚物，从而影响应用的性能。因此，分离和定量共聚物样品中所含的均聚物至关重要。此外，统计共聚物的电荷密度分布是应用的一个重要特征。本工作的目的是通过在酸性条件下（阳离子共聚物）进行毛细管电泳（CE）研究丙烯酸（AA）和二烯丙基二甲基氯化铵（DADMAC）的统计共聚物的特性。为此，根据荷质比（化学组成）进行了自由溶液电泳分离，而与分子量无关。第二个目标是比较非接触式电导检测和间接紫外吸收模式，以定量共聚物样品中存在的 DADMAC 均聚物。还比较了基于中性或正电荷修饰的不同涂层毛细管。间接紫外吸收和非接触电导检测的比较表明，两种检测模式都可用于完整的非紫外吸收 PAA-DADMAC 共聚物的 CE 特性分析。
