Petr Jan, Maier Vítezslav, Horáková Jana, Sevcík Juraj
Department of Analytical Chemistry, Palacký University, Olomouc, Czech Republic.
Electrophoresis. 2006 Dec;27(23):4735-45. doi: 10.1002/elps.200600063.
This work shows the potential of using discontinuous electrolyte systems for the separation of tamsulosin enantiomers by CE. Sulfated beta-cyclodextrin was used as a chiral selector. In acidic electrolytes, sulfated beta-cyclodextrin migrates as an anion and the analyte (tamsulosin) migrates as a cation. Due to this, four experimental arrangements were proposed. These arrangements differ in composition of electrolytes in the inlet compartment, in the capillary and in the outlet compartment. The separation of tamsulosin enantiomers in acetate buffers with sodium and Tris counterions was studied. Simultaneous contactless conductivity detection and UV detection were used for the study of the separation mechanism in these systems. Mobilities of sulfated beta-cyclodextrin were used for the calculation of the time when the analyte migrates through the BGE zone with the selector. The simulation program Simul 4.0 was used for the calculations of the concentration profiles of the electrolyte components dependent on the time of the separation. The mechanism of enantioseparation in these arrangements was suggested.
这项工作展示了使用不连续电解质系统通过毛细管电泳分离坦索罗辛对映体的潜力。硫酸化β-环糊精用作手性选择剂。在酸性电解质中，硫酸化β-环糊精作为阴离子迁移，而分析物（坦索罗辛）作为阳离子迁移。因此，提出了四种实验装置。这些装置在进样区、毛细管和出样区的电解质组成上有所不同。研究了在含有钠和三（羟甲基）氨基甲烷抗衡离子的醋酸盐缓冲液中坦索罗辛对映体的分离。同时使用非接触式电导检测和紫外检测来研究这些系统中的分离机制。硫酸化β-环糊精的迁移率用于计算分析物与选择剂一起迁移通过背景电解质区的时间。使用模拟程序Simul 4.0计算电解质组分的浓度分布随分离时间的变化。提出了这些装置中对映体分离的机制。
