Wei Jun, Li Xue-ling, Sun Yu, Lin Lin, Liu Cai-xia
Department of Obstetrics and Gynecology, Affiliated Shengjing Hospital of China Medical University, Shenyang 110004, China.
Zhonghua Fu Chan Ke Za Zhi. 2011 Aug;46(8):587-90.
To investigate the possible role of perforin (PFN) in the pathogenesis of severe preeclampsia.
Thirty-two cases of severe preeclampsia were included in the study. Thirty-two cases of normal pregnancy were selected as control group in random. The expression of PFN mRNA in the peripheral blood mononuclear cells (PBMC) was detected by reverse transcription (RT)-PCR, and its correlation with mean arterial pressure was analyzed in severe preeclamptic patients. The expression of PFN protein in the decidua was detected by immunohistochemistry.
(1) The expression of PFN mRNA in PBMC:the PFN mRNA level in severe preeclamptic group was 1.19 ± 0.31, and that in normal pregnancy group is 0.82 ± 0.28. The PFN mRNA level in severe preeclamptic group was significantly higher than that of control group (P < 0.01). (2) Correlation analysis: the mean blood pressure in severe preeclampsia group was (133 ± 5) mm Hg (1 mm Hg = 0.133 kPa). There was significant positive correlation between level of PFN mRNA in PBMC and mean blood pressure in severe preeclamptic patients (r = 0.701, P = 0.000). (3) Decidual PFN protein expression:PFN protein was mainly expressed in lymphocytes and the cytoplasm of decidual stromal cells. The positive ratio of PFN in the decidua of severe preeclamptic patients was 84% (27/32), significantly higher than that of control group (53%, 17/32, P < 0.01).
Expression of PFN was significantly increased in severe preeclampsia, and it was of significant positive correlation with mean blood pressure. PFN may participate in the pathogenesis of severe preeclampsia.
探讨穿孔素（PFN）在重度子痫前期发病机制中的可能作用。
本研究纳入32例重度子痫前期患者。随机选取32例正常孕妇作为对照组。采用逆转录（RT）-PCR检测外周血单个核细胞（PBMC）中PFN mRNA的表达，并分析其与重度子痫前期患者平均动脉压的相关性。采用免疫组织化学法检测蜕膜中PFN蛋白的表达。
（1）PBMC中PFN mRNA的表达：重度子痫前期组PFN mRNA水平为1.19±0.31，正常妊娠组为0.82±0.28。重度子痫前期组PFN mRNA水平显著高于对照组（P<0.01）。（2）相关性分析：重度子痫前期组平均血压为（133±5）mmHg（1 mmHg = 0.133 kPa）。重度子痫前期患者PBMC中PFN mRNA水平与平均血压呈显著正相关（r = 0.701，P = 0.000）。（3）蜕膜PFN蛋白表达：PFN蛋白主要表达于淋巴细胞和蜕膜基质细胞的细胞质中。重度子痫前期患者蜕膜中PFN阳性率为84%（27/32），显著高于对照组（53%，17/32，P<0.01）。
重度子痫前期中PFN表达显著增加，且与平均血压呈显著正相关。PFN可能参与重度子痫前期的发病机制。
