Cardiovascular Tissue Engineering Laboratory, Dept. of Surgery, David Geffen School of Medicine, University of California, 10833 Le Conte Avenue, 62-151 CHS, Los Angeles, CA 90095-1741, USA.
Biomaterials. 2012 Mar;33(7):2032-40. doi: 10.1016/j.biomaterials.2011.11.065. Epub 2011 Dec 12.
While stem cell niches in vivo are complex three-dimensional (3D) microenvironments, the relationship between the dimensionality of the niche to its function is unknown. We have created a 3D microenvironment through electrospinning to study the impact of geometry and different extracellular proteins on the development of cardiac progenitor cells (Flk-1(+)) from resident stem cells and their differentiation into functional cardiovascular cells. We have investigated the effect of collagen IV, fibronectin, laminin and vitronectin on the adhesion and proliferation of murine ES cells as well as the effects of these proteins on the number of Flk-1(+) cells cultured in 2D conditions compared to 3D system in a feeder free condition. We found that the number of Flk-1(+) cells was significantly higher in 3D scaffolds coated with laminin or vitronectin compared to colIV-coated scaffolds. Our results show the importance of defined culture systems in vitro for studying the guided differentiation of pluripotent embryonic stem cells in the field of cardiovascular tissue engineering and regenerative medicine.
虽然体内干细胞龛位是复杂的三维（3D）微环境，但龛位的维度与其功能之间的关系尚不清楚。我们通过静电纺丝创建了一个 3D 微环境，以研究微环境的几何形状和不同细胞外蛋白对来源于驻留干细胞的心脏祖细胞（Flk-1(+））发育及其分化为功能性心血管细胞的影响。我们研究了胶原蛋白 IV、纤连蛋白、层粘连蛋白和玻连蛋白对鼠 ES 细胞黏附和增殖的影响，以及这些蛋白对在无饲养细胞条件下 2D 培养条件和 3D 系统中 Flk-1(+）细胞数量的影响。我们发现，与涂有 colIV 的支架相比，涂有层粘连蛋白或玻连蛋白的 3D 支架中 Flk-1(+）细胞的数量明显更高。我们的结果表明，体外定义的培养系统对于研究心血管组织工程和再生医学领域中多能胚胎干细胞的定向分化非常重要。
