Azzar G, Radisson J, Got R
Laboratoire de Biochimie des Membranes (LBTM CNRS-UM 380024), Université Claude Bernard Lyon, Villeurbanne, France.
Parasitol Res. 1990;76(7):578-80. doi: 10.1007/BF00932565.
The addition of [14C]-glucosamine to media of Babesia canis cultures causes the appearance of labeled glycoproteins in the culture supernatants. These radioactive soluble glycoproteins were separated according to their molecular weight by gel filtration and according to their (acidic) pI by preparative electrofocusing. The labeled fractions were then analyzed by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). The results showed three series of glycoproteic antigens. The molecular weights for the three antigens determined by gel filtration and by SDS-PAGE were approximately 100, 40, and 12.5 kDa, and the preparative gel electrofocusing suggested that the antigens focus in the pH range of 3-5.
向犬巴贝斯虫培养物的培养基中添加[14C] - 葡糖胺会导致培养上清液中出现标记的糖蛋白。这些放射性可溶性糖蛋白通过凝胶过滤根据其分子量进行分离，并通过制备性电聚焦根据其（酸性）pI进行分离。然后通过SDS - PAGE（十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳）对标记的组分进行分析。结果显示出三组糖蛋白抗原。通过凝胶过滤和SDS - PAGE测定的三种抗原的分子量分别约为100、40和12.5 kDa，制备性凝胶电聚焦表明这些抗原在pH 3 - 5范围内聚焦。
