Levy M G, Ristic M
Science. 1980 Mar 14;207(4436):1218-20. doi: 10.1126/science.7355284.
The protozoan parasite Babesia bovis, a causative agent of bovine babesiosis, has been continuously cultivated in a settled layer of bovine erythrocytes. Lowered oxygen tension within the layer of host erythrocytes results in a darkening of infected cultures and provides a rapid means of evaluating parasite growth. Deprivation of carbon dioxide causes the merozoites to accumulate in the medium rather than involving new erythrocytes. When separated from the culture, these extraerythrocytic parasites retain their infectivity. Parasites produced in vitro are morphologically identical to parasites from the blood of infected cattle and are susceptible to antibabesial drugs.
原生动物寄生虫牛巴贝斯虫是牛巴贝斯虫病的病原体，已在牛红细胞的沉降层中持续培养。宿主红细胞层内较低的氧张力会导致受感染培养物变黑，并提供一种评估寄生虫生长的快速方法。二氧化碳的缺乏会使裂殖子积聚在培养基中，而不是侵入新的红细胞。当从培养物中分离出来时，这些红细胞外寄生虫仍保持其感染力。体外产生的寄生虫在形态上与感染牛血液中的寄生虫相同，并且对抗巴贝斯虫药物敏感。
