School of Food Science and Environmental Health, Dublin Institute of Technology, Cathal Brugha Street, Dublin 1, Ireland.
Ultrason Sonochem. 2012 May;19(3):582-90. doi: 10.1016/j.ultsonch.2011.11.001. Epub 2011 Nov 23.
The present study optimized the ultrasound assisted extraction (UAE) conditions to maximize the antioxidant activity [Ferric ion Reducing Antioxidant Power (FRAP)], total phenol content (TP) and content of individual polyphenols of extracts from marjoram. Optimal conditions with regard to amplitude of sonication (24.4-61.0 μm) and extraction temperature (15-35 °C) and extraction time (5-15 min) were identified using response surface methodology (RSM). The results showed that the combined treatment conditions of 61 μm, 35 °C and 15 min were optimal for maximizing TP, FRAP, rosmarinic acid, luteolin-7-O-glucoside, apigenin-7-O-glucoside, caffeic acid, carnosic acid and carnosol values of the extracts. The predicted values from the developed quadratic polynomial equation were in close agreement with the actual experimental values with low average mean deviations (E%) ranging from 0.45% to 1.55%. The extraction yields of the optimal UAE were significantly (p < 0.05) higher than solid/liquid extracts. Predicted models were highly significant (p < 0.05) for all the parameters studied with high regression coefficients (R(2)) ranging from 0.58 to 0.989.
本研究通过超声辅助提取（UAE）优化条件，以最大限度地提高迷迭香提取物的抗氧化活性[铁离子还原抗氧化能力（FRAP）]、总酚含量（TP）和单体多酚含量。通过响应面法（RSM）确定了超声幅度（24.4-61.0 μm）、提取温度（15-35°C）和提取时间（5-15 分钟）的最佳条件。结果表明，在 61μm、35°C 和 15 分钟的联合处理条件下，提取液的总酚、FRAP、迷迭香酸、木樨草素-7-O-葡萄糖苷、芹菜素-7-O-葡萄糖苷、咖啡酸、熊果酸和熊果醇的含量最高。所开发的二次多项式方程的预测值与实际实验值非常吻合，平均平均偏差（E%）低至 0.45%至 1.55%。最优 UAE 的提取收率明显（p < 0.05）高于固/液提取。对于所有研究的参数，预测模型均具有高度显著性（p < 0.05），回归系数（R（2））范围为 0.58 至 0.989。
