Iglesias T, Hermida R C, García L, Lodeiro C, Ayala D E, Fernández J R
ETSI Telecomunicación, Universidad de Santiago, Vigo, Spain.
Prog Clin Biol Res. 1990;341A:721-30.
GH concentrations (in ng/ml) were determined by radioimmunoassay in plasma obtained at about 3-hour intervals during a 24-hr sampling span from 40 boys and 14 girls of short stature, 6-14 years of age, living on a diurnal waking (approximately 07:00 to approximately 22:00), nocturnal resting routine. Circadian rhythm parameters were first computed by the single and population mean cosinor fits of a 24-hour cosine curve. Circadian parameter comparisons revealed an intergroup difference between the genders in A (P = .012) and, perhaps, in M (P = .078), but not in 0 (P = .957). In the search for circannual variability, individual circadian Ms were fitted by LLS with a 1 year cosine curve. The LLS harmonic analysis shows a circannual rhythm for the girls (P = .037; M = 8.54; A = 5.38; 0 = -214 degrees from January 1), but not for the boys. Moreover, a 3.5 months prominent component was also found for both the individual circadian Ms (P = .010) and As (P = .012) when all subjects are considered for analysis irrespectively of gender. These infradian characteristics complement circadian differences in GH between boys and girls and must be taken into account in the development of any protocol for studies of GH responses to GH-releasing hormone in children with short stature.
采用放射免疫分析法，对40名6至14岁身材矮小的男孩和14名女孩的血浆生长激素（GH）浓度（单位：ng/ml）进行测定。这些孩子白天清醒（约07:00至约22:00）、夜间休息，在24小时采样期间，每隔约3小时采集一次血样。首先通过对24小时余弦曲线进行单样本和总体均值余弦拟合来计算昼夜节律参数。昼夜节律参数比较显示，在A（P = 0.012）方面，也许在M（P = 0.078）方面，两性之间存在组间差异，但在0方面不存在差异（P = 0.957）。为了寻找年度变化规律，用最小二乘拟合（LLS）将个体的昼夜节律M值与1年余弦曲线进行拟合。最小二乘谐波分析显示，女孩存在年度节律（P = 0.037；M = 8.54；A = 5.38；从1月1日起0 = -214度），但男孩没有。此外，当不考虑性别对所有受试者进行分析时，在个体昼夜节律M值（P = 0.010）和A值（P = 0.012）中还发现了一个3.5个月的显著成分。这些亚日节律特征补充了男孩和女孩之间生长激素的昼夜节律差异，在制定任何关于身材矮小儿童对生长激素释放激素反应的研究方案时都必须予以考虑。
