Garcia L, Hermida R C, Ayala D E, Lodeiro C, Iglesias T
Hospital Materno-Infantil Teresa Herrera, La Coruña, Spain.
Chronobiol Int. 1990;7(3):221-5. doi: 10.3109/07420529009056978.
Cortisol (CT) concentrations (in micrograms/dl) were determined by radioimmunoassay in plasma obtained at about 3-hr intervals during a 24-hr sampling span from 42 boys and 13 girls of short stature (2-4 standard deviations below their peer group mean), and from a reference group of 11 boys and 10 girls with standard stature, before any treatment were administered to the former. Subjects were 11.20 +/- 0.37 years of age at the time of study, and were living on a diurnal waking (approximately 07:30 to approximately 22:30), nocturnal resting routine during sampling, consuming the usual hospital diet. Circadian rhythm parameters were computed separately for each group by the single and population-mean cosinor fits of a 24-hr cosine curve. A comparison of circadian parameters indicates a statistically significant difference in acrophase (phi; P = 0.033) between short and standard children, as well as added differences in rhythm-adjusted mean (M; P = 0.011) and phi (P = 0.035) between boys and girls of short stature. These differences, as well as any other added information from relevant marker rhythms, should be taken into account for the time-specification of therapy before treatment starts in children of short stature.
在对42名身材矮小的男孩和13名身材矮小的女孩（比同龄人平均身高低2至4个标准差）以及11名身材正常的男孩和10名身材正常的女孩组成的参照组在接受任何治疗之前进行的24小时采样期间，每隔约3小时采集一次血浆，通过放射免疫分析法测定血浆中皮质醇（CT）的浓度（微克/分升）。研究时受试者的年龄为11.20±0.37岁，在采样期间保持白天清醒（约07:30至约22:30）、夜间休息的日常作息，食用医院常规饮食。通过对24小时余弦曲线进行单组和总体均值余弦拟合，分别计算每组的昼夜节律参数。昼夜节律参数的比较表明，身材矮小儿童与正常儿童之间的峰值相位（φ；P = 0.033）存在统计学显著差异，身材矮小的男孩和女孩之间在节律调整均值（M；P = 0.011）和φ（P = 0.035）方面也存在额外差异。在开始对身材矮小儿童进行治疗之前，应在治疗时间设定时考虑这些差异以及相关标记节律的任何其他附加信息。
