Ayala D E, Hermida R C, Garcia L, Iglesias T, Lodeiro C
ETSI Telecommunicación, Univ. Vigo, Spain.
Chronobiol Int. 1990;7(3):217-20. doi: 10.3109/07420529009056977.
Growth hormone (GH) concentrations (in ng/ml) were determined by radioimmunoassay, in plasma obtained at about 3-hr intervals during a 24-hr sampling span, from 42 boys and 12 girls of short stature (2-4 standard deviations below their peer group mean), and 13 boys and 9 girls of standard stature. Subjects had 11.20 +/- 0.37 years of age at the time of study, and were living on a diurnal waking (approximately 07:30 to approximately 22:30), nocturnal resting routine during sampling. Analysis of these data by single and population-mean cosinor methods as well as by analysis of variance revealed circadian and ultradian prominent components characterizing most groups. Accordingly, a multiple component analysis was undertaken for data of each group separately, as well as for all subjects. A comparison of circadian parameters indicates similar characteristics between short and standard children, whether one compares boys [P = 0.674, 0.371 and 0.749 for comparison of rhythm adjusted means (M), amplitudes (A) and acrophases (phi), respectively], girls (P = 0.993, 0.914 and 0.397), or all children (P = 0.859, 0.712 and 0.865). Differences are found, however, in circasemidian characteristics as well as in the prominent 8-hr ultradian component documented for the short but not for the standard children. These ultradian components should be taken into consideration in the design and later evaluation of a time-specified treatment of children of short stature.
采用放射免疫分析法测定生长激素（GH）浓度（单位：ng/ml），在24小时采样期间，每隔约3小时采集一次血浆样本，样本来自42名身材矮小的男孩和12名女孩（比同龄人平均身高低2 - 4个标准差）以及13名身材标准的男孩和9名身材标准的女孩。研究时受试者的年龄为11.20±0.37岁，在采样期间保持日间清醒（约07:30至约22:30）、夜间休息的日常作息。通过单因素和总体均值余弦分析法以及方差分析对这些数据进行分析，结果显示大多数组都具有昼夜节律和超日节律的显著成分。因此，分别对每组数据以及所有受试者的数据进行了多成分分析。昼夜节律参数的比较表明，身材矮小和身材标准的儿童具有相似的特征，无论是比较男孩[节律调整均值（M）、振幅（A）和峰相位（φ）的比较P值分别为0.674、0.371和0.749]、女孩（P值分别为0.993、0.914和0.397）还是所有儿童（P值分别为0.859、0.712和0.865）。然而，在短日节律特征以及身材矮小儿童记录到的显著8小时超日节律成分方面存在差异，身材标准的儿童未发现该成分。在设计和随后评估针对身材矮小儿童的定时治疗时，应考虑这些超日节律成分。
