Division of Thoracic and Cardiovascular Surgery, Hannover Medical School, Hannover, Germany.
Surg Today. 2012 Feb;42(3):250-8. doi: 10.1007/s00595-011-0087-3. Epub 2011 Dec 17.
Outcomes following lung transplantation are limited by bronchiolitis obliterans syndrome (BOS). As the number of circulating regulatory T cells (Treg) is lower in lung recipients with BOS than in stable lung recipients, we hypothesized that Treg is also correlated with lung function in the early post-transplantation period.
This prospective study included 18 consecutive patients whose lung function parameters were recorded 3 weeks and 3 months after transplantation, between February and July 2007. Peripheral blood mononuclear cells were stained with anti-CD3, -CD4, -CD8, -CD19, -CD25, -CD28, -CD45RA, -CD45RO, -CD69, -CD127, -CTLA4, and -Foxp3 antibodies and FACS assays were performed. In addition, intracellular cytokines were stained for FACS.
Treg-specific markers (Foxp3, CD127(lo), and CTLA4) in the CD25+ CD4+ population were correlated with both forced expiratory volume in 1 s and forced vital capacity. Th1-cytokine secretion was more dominant in CD4+ CD25+ T cells than in CD4+ CD25- T cells. In contrast, Th2 and Treg cytokine secretion was the dominant response in stable recipients.
The frequency of Treg cells was positively correlated with good lung function in the early period after lung transplantation.
肺移植受者的支气管扩张症闭塞综合征（BOS）会影响其预后。患有 BOS 的肺移植受者的循环调节性 T 细胞（Treg）数量低于稳定的肺移植受者，我们推测 Treg 也与移植后早期的肺功能有关。
本前瞻性研究纳入了 18 例连续患者，其肺功能参数于 2007 年 2 月至 7 月间，在移植后 3 周和 3 个月进行记录。外周血单个核细胞用抗 CD3、CD4、CD8、CD19、CD25、CD28、CD45RA、CD45RO、CD69、CD127、CTLA4 和 Foxp3 抗体染色，并用 FACS 分析。此外，还对细胞内细胞因子进行了 FACS 染色。
CD25+CD4+群体中的 Treg 特异性标志物（Foxp3、CD127(lo)和 CTLA4）与用力呼气量和用力肺活量均相关。与 CD4+CD25-T 细胞相比，CD4+CD25+T 细胞中的 Th1 细胞因子分泌更为明显。相比之下，在稳定的受者中，Th2 和 Treg 细胞因子分泌是主要的反应。
Treg 细胞的频率与肺移植后早期的良好肺功能呈正相关。
