用于鉴别和定量小麦矮缩病毒小麦和大麦株系的实时荧光定量聚合酶链反应检测法
Real-time PCR assay for the discrimination and quantification of wheat and barley strains of Wheat dwarf virus.
作者信息
Gadiou S, Ripl J, Jaňourová B, Jarošová J, Kundu J K
机构信息
Division of Plant Health, Department of Virology, Crop Research Institute, Prague 16106, Czech Republic.
出版信息
Virus Genes. 2012 Apr;44(2):349-55. doi: 10.1007/s11262-011-0699-0. Epub 2011 Dec 16.
A rapid method for detection, discrimination and quantification of wheat and barley strains of wheat dwarf virus (WDV) was successfully developed. The sensitivity of quantification of the wheat and barley strains of WDV ranged from an average of 1.2 × 10(7)-1.2 × 10(2) and from an average of 1.4 × 10(7)-1.4 × 10(4) copies of viral genome, respectively. These standard serial dilutions were applied to plant and vector tissues for virus titer calculations. Both strains of WDV were clearly discriminated by specific probes and melting curve analysis. Both TaqMan(®) and SYBR(®) Green technologies provided accurate and reliable methods for monitoring, detection, discrimination, and quantification of WDV.
一种快速检测、鉴别和定量小麦矮缩病毒（WDV）小麦株系和大麦株系的方法已成功开发。WDV小麦株系和大麦株系的定量灵敏度分别为平均1.2×10⁷ - 1.2×10²个病毒基因组拷贝和平均1.4×10⁷ - 1.4×10⁴个病毒基因组拷贝。这些标准系列稀释液用于植物和载体组织以计算病毒滴度。通过特异性探针和熔解曲线分析可以清楚地区分WDV的两个株系。TaqMan®和SYBR® Green技术都为WDV的监测、检测、鉴别和定量提供了准确可靠的方法。
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