Shibayama T, Kojima H, Ashida M, Hirose S, Sato A, Kamimura T, Hamada C, Shimizu Y, Suzuki S, Ichida F
Gastroenterol Jpn. 1985 Dec;20(6):564-72. doi: 10.1007/BF02774816.
Electron microscopic and virological studies of marmoset liver tissue with acute infection of hepatitis A virus (HAV), especially in the earlier stages of infection, were carried out to characterize the maturation process of HAV. Four marmosets were inoculated intravenously with HAV suspension and sacrificed 1 week, 2 weeks, 3 weeks and 4 weeks after inoculation respectively. Hepatitis A antigen (HAAg) in 10% liver homogenates of marmosets was examined by radioimmunoassay and a large amount of HAAg was detected in the liver homogenate of two marmosets sacrificed 2 weeks and 3 weeks after inoculation respectively. The histodiagnosis of the marmoset sacrificed 2 weeks after HAV inoculation was normal. However, many clusters of virus-like particles about 27 nm in diameter, in both "solid" and "empty" forms were found, mainly in vesicles of Kupffer cells by electron microscopy. In the animal that developed mild hepatitis 3 weeks after inoculation HAV-like particles were found in vesicles of hepatocytes by electron microscopy. By immune electron microscopy using peroxidase-conjugated anti-hepatitis A antibody, HAAg was detected on the particles present within the cytoplasmic vesicles of Kupffer cells or hepatocytes and on the surrounding membrane of the vesicles which contained HAV-like particles.
对感染甲型肝炎病毒（HAV）的狨猴肝脏组织进行了电子显微镜和病毒学研究，特别是在感染的早期阶段，以表征HAV的成熟过程。4只狨猴分别静脉注射HAV悬液，并在接种后1周、2周、3周和4周处死。通过放射免疫分析法检测狨猴10%肝脏匀浆中的甲型肝炎抗原（HAAg），分别在接种后2周和3周处死的两只狨猴的肝脏匀浆中检测到大量HAAg。接种HAV后2周处死的狨猴的组织学诊断为正常。然而，通过电子显微镜观察发现，主要在库普弗细胞的囊泡中存在许多直径约27nm的“实心”和“空心”形式的病毒样颗粒簇。在接种HAV后3周出现轻度肝炎的动物中，通过电子显微镜在肝细胞的囊泡中发现了HAV样颗粒。通过使用过氧化物酶偶联的抗甲型肝炎抗体的免疫电子显微镜观察，在库普弗细胞或肝细胞的细胞质囊泡内的颗粒上以及含有HAV样颗粒的囊泡的周围膜上检测到HAAg。
