Holub B J, Piekarski J
Lipids. 1979 Mar;14(3):309-11. doi: 10.1007/BF02533920.
The present study was conducted to test the selectivity of the microsomal diaclyglycerol kinase (ATP:1,2-diacyl-sn-glycerol-phosphotransferase) from guinea pig brain towards different, 1,2-diacyl-sn-glycerols. The molecular species added to the incubation medium as substrates were a mixture of the 1-[3H]palmitoyl plus 1-[14C]stearoyl homologs of either the monoenoic (2-oleoyl), dienoic (2-linoleoyl), tetraenoic (2-arachidonoyl), or hexaenoic (2-docosahexaenoyl) diacylglycerols. Rates of phosphatidic acid synthesis (1-palmitoy plus 1-stearoyl homologs) with each of the four classes of unsaturated diacylglycerols were not greatly different, although they were moderately higher in the case of the monoenes. No marked enzyme selectivity for either the 1-palmitoyl or 1-stearoyl homolog of the various 1-saturated 2-unsaturated diacylglycerols was apparent. Generally similar results were obtained with the diacylglycerol kinase in rat brain microsomes.
本研究旨在测试豚鼠脑微粒体二酰甘油激酶（ATP：1,2 - 二酰 - sn - 甘油 - 磷酸转移酶）对不同的1,2 - 二酰 - sn - 甘油的选择性。作为底物添加到孵育介质中的分子种类是单烯酸（2 - 油酰基）、二烯酸（2 - 亚油酰基）、四烯酸（2 - 花生四烯酰基）或六烯酸（2 - 二十二碳六烯酰基）二酰甘油的1 - [³H]棕榈酰基加1 - [¹⁴C]硬脂酰基同系物的混合物。四种不饱和二酰甘油中的每一种的磷脂酸合成速率（1 - 棕榈酰基加1 - 硬脂酰基同系物）差异不大，尽管单烯酸类的速率略高。对于各种1 - 饱和2 - 不饱和二酰甘油的1 - 棕榈酰基或1 - 硬脂酰基同系物，没有明显的酶选择性。在大鼠脑微粒体中的二酰甘油激酶也获得了大致相似的结果。
