狒狒（Papio cynocephalus）组织中甘油二酯激酶的不同花生四烯酰特异性和非特异性同工酶分布。
Distribution of distinct arachidonoyl-specific and non-specific isoenzymes of diacylglycerol kinase in baboon (Papio cynocephalus) tissues.
作者信息
Lemaitre R N, King W C, MacDonald M L, Glomset J A
机构信息
Howard Hughes Medical Institute, Department of Biochemistry, University of Washington, Seattle 98195.
出版信息
Biochem J. 1990 Feb 15;266(1):291-9. doi: 10.1042/bj2660291.
We investigated the diacyglycerol kinase species present in several baboon tissues using the substrates sn-1-stearoyl-2-arachidonoyl diacylglycerol and sn-1,2-didecanoyl diacylglycerol. Chromatography of octyl glucoside extracts of the baboon (Papio cynocephalus papio) tissues on hydroxyapatite columns revealed the presence of three diacylglycerol kinase species with different substrate preferences. One species markedly 'preferred' the substrate sn-1-stearoyl-2-arachidonoylglycerol, the two other species preferred sn-1,2-didecanoylglycerol. Measurement of the activity of the baboon brain diacylglycerol kinases toward diacylglycerols with a range of different fatty acid chains revealed a strict preference of the arachidonoyl diacylglycerol kinase for sn-1-acyl-2-arachidonoyl diacylglycerol, whereas the other enzymes showed no preference toward several long-chain-fatty-acid-containing diacylglycerols. The arachidonoyl diacylglycerol kinase was particularly abundant in brain and testis, whereas liver was practically devoid of this enzyme. The arachidonoyl diacylglycerol kinase from baboon brain was found to be predominantly associated with the particulate fraction and exhibited an apparent molecular mass of 130 kDa.
我们使用底物sn-1-硬脂酰-2-花生四烯酰二酰甘油和sn-1,2-二十二烷酰二酰甘油，研究了几种狒狒组织中存在的二酰甘油激酶种类。狒狒（豚尾狒狒）组织的辛基葡糖苷提取物在羟基磷灰石柱上进行色谱分析，结果显示存在三种具有不同底物偏好的二酰甘油激酶种类。其中一种种类明显“偏好”底物sn-1-硬脂酰-2-花生四烯酰甘油，另外两种种类则偏好sn-1,2-二十二烷酰甘油。对狒狒脑二酰甘油激酶对一系列具有不同脂肪酸链的二酰甘油的活性进行测定，结果显示花生四烯酰二酰甘油激酶对sn-1-酰基-2-花生四烯酰二酰甘油有严格偏好，而其他酶对几种含长链脂肪酸的二酰甘油没有偏好。花生四烯酰二酰甘油激酶在脑和睾丸中特别丰富，而肝脏中几乎没有这种酶。发现来自狒狒脑的花生四烯酰二酰甘油激酶主要与颗粒部分相关，其表观分子量为130 kDa。
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