Muro Tomonori, Fujihara Junko, Imamura Shinji, Nakamura Hiroaki, Kimura-Kataoka Kaori, Toga Tomoko, Iida Reiko, Yasuda Toshihiro, Takeshita Haruo
Department of Legal Medicine, Faculty of Medicine, Shimane University, Izumo 693-8501, Japan.
Leg Med (Tokyo). 2012 Jan;14(1):47-50. doi: 10.1016/j.legalmed.2011.10.002. Epub 2011 Dec 16.
ABO grouping of biological specimens is informative for identifying victims and narrowing down suspects. In Japan and elsewhere, ABO grouping as well as DNA profiling plays an essential role in crime investigations. In the present study, we developed a new method for ABO genotyping using allele-specific primers and real-time PCR. The method allows for the detection of three single nucleotide polymorphisms (SNPs) at nucleotide positions 261, 796, and 803 in the ABO gene and the determination of six major ABO genotypes. This method required less than 2 h for accurate ABO genotyping using 2.0 ng of DNA. This method could be applicable for rapid and simple screening of forensic samples.
生物样本的ABO血型分组有助于识别受害者并缩小嫌疑人范围。在日本及其他地区，ABO血型分组和DNA分析在犯罪调查中都起着至关重要的作用。在本研究中，我们开发了一种使用等位基因特异性引物和实时PCR进行ABO基因分型的新方法。该方法能够检测ABO基因中第261、796和803位核苷酸处的三个单核苷酸多态性（SNP），并确定六种主要的ABO基因型。使用2.0 ng DNA进行准确的ABO基因分型，该方法所需时间不到2小时。此方法可用于法医样本的快速简易筛查。
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