Mohamed Abdularahman B O, Hindawi Salwa Ibrahim, Al-Harthi Sameer, Alam Qamre, Alam Mohammad Zubair, Haque Absarul, Ahmad Waseem, Damanhouri Ghazi A
Department of Pharmacology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
Faculty of Medicine, Department of Hematology, King Abdulaziz University Hospital, Jeddah, Saudi Arabia.
Blood Res. 2016 Dec;51(4):274-278. doi: 10.5045/br.2016.51.4.274. Epub 2016 Dec 23.
Characterization of the ABO blood group at the phenotype and genotype levels is clinically essential for transfusion, forensics, and population studies. This study elucidated ABO phenotypes and genotypes, and performed an evaluation of their distribution in individuals from the western region of Saudi Arabia.
One-hundred and seven samples underwent standard serological techniques for ABO blood group phenotype analysis. alleles and genotypes were identified using multiplex polymerase chain reaction, and electrophoretic analysis was performed to evaluate the highly polymorphic locus.
A phenotype distribution of 37.4%, 30.8%, 24.3%, and 7.5% was found for blood groups O, A, B, and AB respectively in our study cohort. Genotype analysis identified 10 genotype combinations with the and genotypes being the most frequent with frequencies of 33.6% and 14.95%, respectively. Common genotypes such as , , , , and were not detected. Similarly, the rare genotypes, , , and were not found in our cohort. The most frequently observed allele was O02 (35.98%) followed by the allele (17.76%). Furthermore, our findings are discussed in reference to allele and genotype frequencies found in other ethnic groups.
The study has a significant implication on the management of blood bank and transfusion services in Saudi Arabian patients.
在表型和基因型水平上对ABO血型进行鉴定在输血、法医和群体研究中具有重要的临床意义。本研究阐明了ABO血型的表型和基因型，并对沙特阿拉伯西部地区个体的ABO血型分布进行了评估。
107份样本采用标准血清学技术进行ABO血型表型分析。使用多重聚合酶链反应鉴定等位基因和基因型，并进行电泳分析以评估高度多态性位点。
在我们的研究队列中，血型O、A、B和AB的表型分布分别为37.4%、30.8%、24.3%和7.5%。基因型分析确定了10种基因型组合，其中O01/O02和A101/O02基因型最为常见，频率分别为33.6%和14.95%。未检测到常见基因型，如O01/O01、A101/A101、B01/B01、A101/B01和A201/O02。同样，我们的队列中也未发现罕见基因型，如A201/A201、A201/B01和B(A)01/O02。观察到的最常见等位基因为O02（35.98%），其次是A101等位基因（17.76%）。此外，我们还参考其他种族群体的A、B等位基因和基因型频率对研究结果进行了讨论。
本研究对沙特阿拉伯患者血库和输血服务的管理具有重要意义。
