College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Int Immunopharmacol. 2012 Jan;12(1):241-8. doi: 10.1016/j.intimp.2011.11.017. Epub 2011 Dec 13.
Alpinetin, one of the main constituents of the seeds of Alpinia katsumadai Hayata, belonging to flavonoids, has been known to exhibit antibacterial, anti-inflammatory and other important therapeutic activities. The purpose of this study was to investigate the protection of alpinetin on inflammation in Lipopolysaccharide (LPS) stimulated Raw 264.7 cells and LPS induced vivo lung injury model. The effects of alpinetin on pro-inflammatory cytokines and signaling pathways were analyzed by enzyme-linked immunosorbent assay and Western blot. The results showed that alpinetin markedly inhibited the LPS- induced TNF-α, IL-6 and IL-1β production both in vitro and vivo. Furthermore, alpinetin blocked the phosphorylation of IκBα protein, p65, p38 and extracellular signal-regulated kinase (ERK) in LPS stimulated RAW 264.7 cells. From in vivo study, it was also observed that alpinetin attenuated lung histopathologic changes in mouse models. These results suggest that alpinetin potentially decreases the inflammation in vitro and vivo, and might be a therapeutic agent against inflammatory diseases.
山姜素，山姜属植物的种子中的主要成分之一，属于类黄酮，已被证明具有抗菌、抗炎等重要的治疗作用。本研究旨在探讨山姜素对脂多糖（LPS）刺激的 Raw 264.7 细胞和 LPS 诱导的体内肺损伤模型的炎症的保护作用。通过酶联免疫吸附试验和 Western blot 分析了山姜素对促炎细胞因子和信号通路的影响。结果表明，山姜素显著抑制 LPS 诱导的 TNF-α、IL-6 和 IL-1β的产生，无论是在体外还是体内。此外，山姜素阻断了 LPS 刺激的 RAW 264.7 细胞中 IκBα 蛋白、p65、p38 和细胞外信号调节激酶（ERK）的磷酸化。从体内研究中还观察到，山姜素减轻了小鼠模型中的肺组织病理变化。这些结果表明，山姜素可能在体外和体内减轻炎症，并且可能是一种治疗炎症性疾病的药物。
