Department of Pharmaceutics, Institute of Technology, Banaras Hindu University, Varanasi, India.
Fitoterapia. 2012 Mar;83(2):395-401. doi: 10.1016/j.fitote.2011.12.008. Epub 2011 Dec 9.
Bergenin, a major constituent of Caesalpinia digyna Rottler (Leguminosae) was isolated from its roots and was characterized by comparing its melting point and spectroscopic data (IR, (1)H, (13)C, Mass Spectra) with standard bergenin. Isolated bergenin was then evaluated for antidiabetic (Type 2) activity in streptozotocin (STZ)-nicotinamide induced diabetic rats. Bergenin was administered at doses of 2.5, 5, and 10 mg/kg; p.o. to normal rats which were subjected to oral glucose tolerance test (OGTT). Bergenin at same dose level was given to diabetic rats and fasting blood glucose level was estimated on 0th, 7th and 14th day of treatment while plasma lipids, antioxidant enzymes and liver glycogen level in diabetic rats were estimated on 14th day of treatment followed by histopathological studies of pancreas. Bergenin at 10mg/kg; p.o. was found to reduce blood glucose level significantly in OGTT (P<0.01) while it showed a significant reduction in fasting blood glucose level in diabetic rats at same dose level only on 14th day of treatment. Bergenin in all dose levels reversed plasma lipid (reduced elevated TC, LDL-C and increased HDL-C level) profile to normal values except TG. However, bergenin showed no significant effect on liver glycogen at all dose level. The decrease in lipid peroxides and increase in superoxide dismutase (SOD) and catalase (CAT) in liver illustrated the antioxidant potential of bergenin. Histopathological studies demonstrated the regenerative effect of bergenin on pancreatic β cells. Hence, bergenin isolated from C. digyna possesses significant antidiabetic, hypolipidemic and antioxidant activity in Type 2 diabetic rats.
从该植物的根部分离出了主要成分之一的毛蕊异黄酮，并通过比较其熔点和光谱数据（IR、（1）H、（13）C、质谱）与标准毛蕊异黄酮对其进行了鉴定。然后，对分离得到的毛蕊异黄酮进行了 2-型糖尿病（STZ-烟酰胺诱导的糖尿病）大鼠的抗糖尿病（2 型）活性评价。毛蕊异黄酮以 2.5、5 和 10mg/kg 的剂量；p.o. 给予正常大鼠，然后进行口服葡萄糖耐量试验（OGTT）。在第 7 天和第 14 天治疗时，对糖尿病大鼠给予相同剂量水平的毛蕊异黄酮，并测定空腹血糖水平，同时在第 14 天治疗时测定糖尿病大鼠的血浆脂质、抗氧化酶和肝糖原水平，并进行胰腺组织病理学研究。结果发现，毛蕊异黄酮以 10mg/kg；p.o. 可显著降低 OGTT 中的血糖水平（P<0.01），而在相同剂量水平下，仅在第 14 天治疗时，可显著降低糖尿病大鼠的空腹血糖水平。毛蕊异黄酮在所有剂量水平下均可使血浆脂质（降低升高的 TC、LDL-C 和升高的 HDL-C 水平）谱恢复正常，除 TG 外。然而，毛蕊异黄酮在所有剂量水平下对肝糖原均无显著影响。肝脏中脂质过氧化物的减少和超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的增加说明了毛蕊异黄酮的抗氧化潜力。组织病理学研究表明，毛蕊异黄酮对胰腺β细胞具有再生作用。因此，从 C. digyna 中分离出的毛蕊异黄酮在 2 型糖尿病大鼠中具有显著的抗糖尿病、降血脂和抗氧化活性。
