Singh Jitender, Kumar Ashwani, Sharma Anupam
University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh 160014, India.
University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh 160014, India.
J Ethnopharmacol. 2017 Jan 4;195:182-187. doi: 10.1016/j.jep.2016.11.016. Epub 2016 Nov 12.
Caesalpinia digyna Rottler (Caesalpiniaceae) roots have been used traditionally for soothing nerves, as nervine tonic and febrifuge. The aim of the present study was to isolate the antianxiety constituent(s) from C. digyna roots following bioactivity guided fractionation approach.
Bioactive fraction F4 was subjected to column chromatography to get three sub-fractions (F4.1-F4.3) and a compound CD2. CD1 and CD2 were characterized using standard spectral techniques. Three fractions (F4.1-F4.3) were evaluated for antianxiety activity in mice using EPM. An effective dose of the antianxiety principle was optimized, and its activity was, further evaluated using open field and mirror chamber tests.
CD1 and CD2 characterized as bergenin, exhibited significant antianxiety activity at 80mg/kg, po which was statistically comparable to that of diazepam (2mg/kg, po). However, F4.1-F4.3 were observed to be devoid of antianxiety activity. Bergenin also exhibited significant antianxiety activity in open field and mirrored chamber tests.
Bergenin, isolated from roots of C. digyna, exhibits significant antianxiety activity at 80mg/kg, po in three different models for evaluating antianxiety activity. This activity of bergenin is being reported for the first time.
孪叶苏木（苏木科）根传统上用于舒缓神经，作为神经滋补剂和退烧药。本研究的目的是采用生物活性导向分级分离方法从孪叶苏木根中分离出抗焦虑成分。
生物活性组分F4经柱色谱分离得到三个亚组分（F4.1 - F4.3）和一种化合物CD2。使用标准光谱技术对CD1和CD2进行表征。使用高架十字迷宫对三个亚组分（F4.1 - F4.3）在小鼠中的抗焦虑活性进行评估。优化抗焦虑成分的有效剂量，并使用旷场试验和镜像箱试验进一步评估其活性。
鉴定为岩白菜素的CD1和CD2在80mg/kg灌胃给药时表现出显著的抗焦虑活性，这在统计学上与地西泮（2mg/kg灌胃给药）相当。然而，观察到F4.1 - F4.3没有抗焦虑活性。岩白菜素在旷场试验和镜像箱试验中也表现出显著的抗焦虑活性。
从孪叶苏木根中分离出的岩白菜素在80mg/kg灌胃给药时，在三种不同的抗焦虑活性评估模型中表现出显著的抗焦虑活性。岩白菜素的这种活性首次被报道。
