Vinijsanun A, Martin L, Wang D Y, Fantl V E
Department of Physiology and Pharmacology, University of Queensland, St Lucia, Australia.
Reprod Fertil Dev. 1990;2(4):395-405. doi: 10.1071/rd9900395.
A monoclonal antibody against progesterone (11P27) given on Day 2 of pregnancy interrupted pregnancy in BALB/c mice but not in BCF1 mice. The reason for this strain difference remains unclear, although it may involve discrimination by the recipient's immune system. The effects in BALB/c mice were reversed by progestin treatment. A dose of 5 nmol, which completely blocked implantation, had no significant effects on embryo transport and development on Day 4. A dose of 10 nmol did not increase the proportion of abnormal embryos, even though it accelerated tubal transport and increased embryo loss. No tubal retention was evident; most remaining embryos reached the uterus at the normal time. The transformation of morulae to blastocysts was only slightly delayed in 11P27-treated mice, and transfer experiments showed no decrease in embryo viability. The antibody appeared to act by blocking actions of endogenous progesterone on the uterus: uteri of 11P27-treated mice failed to develop a decidual cell reaction to intrauterine oil, and embryos from untreated donors failed to implant in 11P27-treated recipients. Antagonism of progesterone by antibody treatment prevented implantation in BALB/c mice apparently by actions on the uterus rather than on the embryo.
在妊娠第2天给予抗孕酮单克隆抗体（11P27）可中断BALB/c小鼠的妊娠，但对BCF1小鼠无效。尽管这种品系差异的原因可能涉及受体免疫系统的识别，但仍不清楚。在BALB/c小鼠中的作用可通过孕激素治疗逆转。5 nmol的剂量可完全阻断着床，但对第4天的胚胎转运和发育无显著影响。10 nmol的剂量虽加速了输卵管转运并增加了胚胎丢失，但并未增加异常胚胎的比例。未见输卵管滞留现象；大多数剩余胚胎按时到达子宫。在经11P27处理的小鼠中，桑椹胚向囊胚的转化仅略有延迟，移植实验表明胚胎活力未降低。该抗体似乎通过阻断内源性孕酮对子宫的作用而起作用：经11P27处理的小鼠子宫未能对子宫内注射油产生蜕膜细胞反应，未处理供体的胚胎也未能在经11P27处理的受体中着床。抗体治疗对孕酮的拮抗作用显然是通过作用于子宫而非胚胎来阻止BALB/c小鼠着床。
