利用光热光学锁相光学相干显微镜对活细胞中的金纳米颗粒进行快速三维成像。

Fast three-dimensional imaging of gold nanoparticles in living cells with photothermal optical lock-in Optical Coherence Microscopy.

作者信息

Pache Christophe, Bocchio Noelia L, Bouwens Arno, Villiger Martin, Berclaz Corinne, Goulley Joan, Gibson Matthew I, Santschi Christian, Lasser Theo

机构信息

Laboratoire d’Optique Biom´edicale, Ecole Polytechnique F´ed´erale de Lausanne, Lausanne, Switzerland.

出版信息

Opt Express. 2012 Sep 10;20(19):21385-99. doi: 10.1364/OE.20.021385.

DOI:10.1364/OE.20.021385

PMID:23037262

Abstract

We introduce photothermal optical lock-in Optical Coherence Microscopy (poli-OCM), a volumetric imaging technique, which combines the depth sectioning of OCM with the high sensitivity of photothermal microscopy while maintaining the fast acquisition speed inherent to OCM. We report on the detection of single 40 nm gold particles with a 0.5 μm lateral and 2 μm axial resolution over a 50 μm depth of field and the three-dimensional localization of gold colloids within living cells. In combination with intrinsic sample contrast measured with dark-field OCM, poli-OCM offers a versatile platform for functional cell imaging.

摘要

我们介绍了光热光学锁相光学相干显微镜（poli-OCM），这是一种体积成像技术，它将光学相干显微镜（OCM）的深度切片与光热显微镜的高灵敏度相结合，同时保持了OCM固有的快速采集速度。我们报告了在50μm景深范围内以0.5μm横向分辨率和2μm轴向分辨率检测单个40nm金颗粒以及在活细胞内对金胶体进行三维定位的情况。结合暗场OCM测量的固有样品对比度，poli-OCM为功能细胞成像提供了一个多功能平台。