Cell Biology Laboratory, Division of Pharmacology, Indian Institute of Integrative Medicine, Jammu Tawi, Jammu and Kashmir-80001, India.
Phytother Res. 2012 Aug;26(8):1156-65. doi: 10.1002/ptr.3684. Epub 2011 Dec 19.
Despite chlorogenic acid (CGA) being widely present in nature, particularly in the human diet, there is very little information regarding its pharmacological activities. The present investigation was carried out to investigate the antiarthritic activities of this compound in adjuvant induced-arthritis in male Wistar rats, and to explore the underlying mechanisms of actions in view of immunological responses. We observed that CGA effectively controlled the total (CD3) and differentiated (CD4 and CD8) T cells count at the dose of 40 mg/kg. We also assessed the effect on co-stimulatory molecules (CD28, CD80/86) and found that CGA efficiently suppressed CD80/86 but failed to bring any changes in the CD28 count, whereas ibuprofen (standard drug) resulted in highly significant inhibition of both. We next examined the effect on CD4⁺ T cells specific Th1/Th2 cytokines by flow cytometry and observed that CGA suppressed the Th1 cytokines in a highly significant manner but elevated Th2 cytokines with dose dependence. Results of the present investigation suggest that CGA is a potent antiarthritic agent.
尽管绿原酸（CGA）广泛存在于自然界中，特别是在人类饮食中，但关于其药理活性的信息却很少。本研究旨在探讨该化合物在佐剂诱导的雄性 Wistar 大鼠关节炎中的抗关节炎活性，并鉴于免疫反应，探讨其作用机制。我们观察到 CGA 能有效控制总（CD3）和分化（CD4 和 CD8）T 细胞计数，剂量为 40mg/kg。我们还评估了对共刺激分子（CD28、CD80/86）的影响，发现 CGA 能有效抑制 CD80/86，但对 CD28 计数没有任何影响，而布洛芬（标准药物）则能显著抑制这两种分子。接下来，我们通过流式细胞术检测了 CGA 对 CD4+T 细胞特异性 Th1/Th2 细胞因子的影响，结果发现 CGA 能显著抑制 Th1 细胞因子，但能剂量依赖性地升高 Th2 细胞因子。本研究结果表明，CGA 是一种有效的抗关节炎药物。
