USDA ARS Livestock Issues Research Unit, Lubbock, TX 79403, USA.
Innate Immun. 2012 Aug;18(4):592-601. doi: 10.1177/1753425911428964. Epub 2011 Dec 16.
The study examined the effect of chromium supplementation on the response of steers to an LPS challenge. Steers received a premix that added 0 (control; n = 10) or 0.2 mg/kg of chromium (n = 10) to the total diet on a dry matter basis for 56 d. Steers were fitted with jugular catheters and rectal temperature (RT) recording devices on d 52. Blood samples were collected and sickness behavior scores assigned to each steer relative to an LPS challenge (0.5 µg/kg) on d 55. Pre-LPS RT were greater in chromium-supplemented than in control steers. Post-LPS RT increased in both treatments, with control steers producing a greater change in RT than chromium-supplemented steers. Sickness behavior scores were greater in control than in chromium-supplemented steers post-LPS (P = 0.03). Cortisol concentrations did not differ between treatments pre-LPS. Post-LPS cortisol concentrations increased but did not differ due to treatment. Concentrations of IL-4 increased post-LPS but were not affected by treatment pre- or post-LPS. Treatment did not affect pre-LPS TNF-α or IFN-γ. Post-LPS TNF-α and IFN-γ increased in both treatments, with chromium-supplemented steers producing greater TNF-α (P = 0.005) and IFN-γ (P = 0.004) than control steers. Pre-LPS IL-6 was greater (P = 0.027) in chromium-supplemented steers than in control steers. Post-LPS IL-6 increased in both treatments and was greater (P < 0.001) in chromium-supplemented than in control steers. These data suggest that chromium supplementation enhances the acute phase response of steers to an LPS challenge, which may expedite recovery.
本研究旨在探讨铬补充对 LPS 应激下肉牛反应的影响。在 56 天的干物质基础日粮中，肉牛分别添加 0（对照组，n = 10）或 0.2mg/kg 的铬（n = 10）作为预混料。在第 52 天，肉牛安装颈静脉导管和直肠温度（RT）记录设备。在第 55 天，每头肉牛注射 LPS（0.5μg/kg）后，采集血液样本并进行疾病行为评分。与对照组相比，补充铬的肉牛在 LPS 前的 RT 更高。两种处理方式的 RT 均在 LPS 后增加，对照组的 RT 变化大于补充铬的肉牛。与对照组相比，补充铬的肉牛在 LPS 后疾病行为评分更高（P = 0.03）。LPS 前，两种处理方式的皮质醇浓度没有差异。LPS 后，皮质醇浓度增加，但由于处理方式不同而没有差异。IL-4 浓度在 LPS 后增加，但不受处理方式的影响。处理方式不影响 LPS 前 TNF-α或 IFN-γ。两种处理方式的 TNF-α和 IFN-γ在 LPS 后均增加，补充铬的肉牛产生的 TNF-α（P = 0.005）和 IFN-γ（P = 0.004）均大于对照组。LPS 前，补充铬的肉牛的 IL-6 更高（P = 0.027）。两种处理方式的 IL-6 在 LPS 后均增加，补充铬的肉牛的 IL-6 高于对照组（P < 0.001）。这些数据表明，铬补充增强了肉牛对 LPS 应激的急性期反应，可能加速恢复。
