Meyer Jennifer, Kononov Susanne Ursula, Grindler Sandra, Tröscher-Mußotter Johanna, Alaedin Mohamad Taher, Frahm Jana, Hüther Liane, Kluess Jeannette, Kersten Susanne, von Soosten Dirk, Meyer Ulrich, Most Erika, Eder Klaus, Sauerwein Helga, Seifert Jana, Huber Korinna, Wegerich Anja, Rehage Jürgen, Dänicke Sven
Institute of Animal Nutrition, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Bundesallee 37, 38116 Braunschweig, Germany.
Institute of Animal Science, Functional Anatomy of Livestock, University of Hohenheim, Fruwirthstraße 35, 70593 Stuttgart, Germany.
Animals (Basel). 2021 Jan 10;11(1):136. doi: 10.3390/ani11010136.
l-carnitine plays an important role in energy metabolism through supporting the transport of activated fatty acids to the subcellular site of β-oxidation. An acute phase reaction (APR) is known as an energy consuming process. Lipopolysaccharides (LPS) are often used in animal models to study intervention measures during innate immune responses such as APR. Thus, the aim of the study was to investigate the effects of dietary l-carnitine supplementation during an LPS-induced APR in mid-lactating German Holstein cows. Animals were assigned to a control (CON, n = 26) or l-carnitine group (CAR, = 27, 25 g rumen-protected l-carnitine/cow/d) and received an intravenous injection of LPS (0.5 μg/kg body weight) at day 111 post-partum. Blood samples were collected from day 1 pre-injection until day 14 post-injection (pi). From 0.5 h pi until 72 h pi blood samplings and clinical examinations were performed in short intervals. Clinical signs of the APR were not altered in group CAR except rumen motility which increased at a lower level compared to the CON group after a period of atonia. Group CAR maintained a higher insulin level compared to group CON even up to 72 h pi which might support glucose utilization following an APR.
左旋肉碱通过支持活化脂肪酸转运至β-氧化的亚细胞部位，在能量代谢中发挥重要作用。急性期反应（APR）是一个耗能过程。脂多糖（LPS）常用于动物模型，以研究诸如APR等先天免疫反应期间的干预措施。因此，本研究的目的是调查在产奶中期的德国荷斯坦奶牛中，LPS诱导的APR期间补充日粮左旋肉碱的效果。将动物分为对照组（CON，n = 26）或左旋肉碱组（CAR，n = 27，25 g瘤胃保护性左旋肉碱/头/天），并在产后第111天静脉注射LPS（0.5 μg/kg体重）。从注射前1天至注射后14天（pi）采集血样。从注射后0.5小时至72小时，每隔一段时间进行血样采集和临床检查。CAR组APR的临床症状没有改变，只是瘤胃蠕动在一段时间的弛缓后有所增加，但与CON组相比增加幅度较小。与CON组相比，CAR组甚至在注射后72小时仍保持较高的胰岛素水平，这可能有助于APR后的葡萄糖利用。
