School of Industrial Technology, Universiti Sains Malaysia, Minden - 11800, Pulau Pinang, Malaysia.
Nat Prod Res. 2012;26(22):2155-8. doi: 10.1080/14786419.2011.633083. Epub 2011 Dec 19.
The leaves of Cinnamomum iners (Reinw. ex Blume-Lauraceae) have been refluxed successively with chloroform and alcohol to get chloroform extract and alcoholic extract. Both the extracts have been assayed for cytotoxicity against human colorectal tumour cells. The chloroform extract exhibited significant cytotoxicity with IC(50) 31 µg mL(-1) (p < 0.01). However, ethanol extract was found to be much less cytotoxic with IC(50) > 200 µg mL(-1). The chloroform extract has been further proceeded for chemical analysis by GC-TOFMS and 178 components were identified including acids, amines, amides, aldehydes, alcohols, esters, benzene derivatives, bicyclic compounds, terpenes, hydrocarbons, naphthalene derivatives, furan derivatives, azulenes, etc. Nine components representing 51.73% of the total chloroform extract were detected as major components. Caryophyllene (14.41%) and Eicosanoic acid ethyl ester (12.17%) are the most prominent components of the chloroform extract. β-Caryophyllene (14.41%) as most abundant compound supports potent cytotoxicity as shown by chloroform extract.
山肉桂（Reinw. ex Blume-樟科）的叶子已用氯仿和乙醇回流提取，得到氯仿提取物和醇提物。这两种提取物均已用于检测对人结直肠肿瘤细胞的细胞毒性。氯仿提取物表现出显著的细胞毒性，IC（50）为 31μg mL（-1）（p<0.01）。然而，乙醇提取物的细胞毒性要小得多，IC（50）>200μg mL（-1）。氯仿提取物已进一步通过 GC-TOFMS 进行化学分析，鉴定出 178 种成分，包括酸、胺、酰胺、醛、醇、酯、苯衍生物、双环化合物、萜类、烃、萘衍生物、呋喃衍生物、薁等。9 种成分代表了总氯仿提取物的 51.73%，被检测为主要成分。石竹烯（14.41%）和二十烷酸乙酯（12.17%）是氯仿提取物中最显著的成分。β-石竹烯（14.41%）作为最丰富的化合物，支持氯仿提取物所表现出的强大细胞毒性。
