Faculty of Pharmacy, Department of Pharmacognosy, Cairo University, Kasr el-Aini Street, Cairo 11561, Egypt.
Nat Prod Res. 2012;26(22):2057-62. doi: 10.1080/14786419.2011.606219. Epub 2011 Aug 17.
Ten compounds, neopulchellin (1), 6α- hydroxyneopulchellin (2), β-sitosterol-3-O-β-D-glucoside (3), apigenin (4), quercitin (5), eupafolin (6), kaempferol-3-methoxy-7-O-α-L-rhamnoside (7), apigenin-7-O-β-D-glucopyranoside (8), α-amyrin (9) and β-sitosterol (10), were isolated from the leaves of Gaillardia aristata by applying bioassay guided fractionation. The cytotoxicity was traced against two human cancer cell lines (breast (MCF7) and colon (HCT116)). The highest cytotoxicity was revealed by compounds 1 and 2 (isolated from chloroform extract); with IC(50) values of 0.43, 0.32 µg mL(-1) against MCF7 and 0.46, 0.34 µg mL(-1) against HCT116, respectively. Compounds 9 and 10 (isolated from the n-hexane extract) exhibited lower IC(50) values of 3.05, 2.35 µg mL(-1) against MCF7 and 3.05, 2.35 µg mL(-1) against HCT116, respectively, while compounds 4-7 obtained from the ethyl acetate extract revealed the lowest cytotoxicity. Identification of the aforementioned compounds was carried out on the basis of their physico-chemical properties and spectral analysis (UV, EI/MS, 1D and 2D).
从矢车天人菊的叶子中通过应用生物测定导向的分段分离得到了 10 种化合物，分别为 neo pulchellin（1）、6α-羟基 neo pulchellin（2）、β-谷甾醇-3-O-β-D-葡萄糖苷（3）、芹菜素（4）、槲皮素（5）、eupafolin（6）、kaempferol-3-甲氧基-7-O-α-L-鼠李糖苷（7）、芹菜素-7-O-β-D-吡喃葡萄糖苷（8）、α-香树脂醇（9）和β-谷甾醇（10）。通过对两种人类癌细胞系（乳腺癌（MCF7）和结肠癌（HCT116））进行细胞毒性追踪，发现化合物 1 和 2（从氯仿提取物中分离得到）具有最高的细胞毒性；对 MCF7 的 IC50 值分别为 0.43、0.32μgmL(-1)，对 HCT116 的 IC50 值分别为 0.46、0.34μgmL(-1)。化合物 9 和 10（从正己烷提取物中分离得到）对 MCF7 的 IC50 值分别为 3.05、2.35μgmL(-1)，对 HCT116 的 IC50 值分别为 3.05、2.35μgmL(-1)，而从乙酸乙酯提取物中得到的化合物 4-7 则显示出最低的细胞毒性。上述化合物的鉴定是基于它们的物理化学性质和光谱分析（UV、EI/MS、1D 和 2D）进行的。
