利用 rpoB 基因序列分析对克罗诺杆菌属进行系统发育鉴定。
Use of rpoB gene sequence analysis for phylogenetic identification of Cronobacter species.
机构信息
College of Food Science and Technology, Nanjing Agricultural University, Key Laboratory of Food Processing and Quality Control, Ministry of Agriculture of China, 1 Weigang Nanjing 210095, PR China.
出版信息
J Microbiol Methods. 2012 Feb;88(2):316-8. doi: 10.1016/j.mimet.2011.12.002. Epub 2011 Dec 13.
Here, we report the RNA polymerase beta-subunit gene (rpoB) as a new molecular marker for the identification of the Cronobacter species. The results indicated that members of the Cronobacter genus are more easily discriminated by rpoB sequencing than 16S rRNA sequencing, and reliable identification could be achieved by rpoB gene sequence comparison.
在这里，我们报告 RNA 聚合酶β亚基基因（rpoB）作为鉴定克罗诺杆菌属种的新分子标记。结果表明，与 16S rRNA 测序相比，rpoB 测序更易于区分克罗诺杆菌属成员，并且通过 rpoB 基因序列比较可以实现可靠的鉴定。
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