Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague 4-Krč, Czech Republic.
Folia Microbiol (Praha). 2014 Jan;59(1):63-8. doi: 10.1007/s12223-013-0266-2. Epub 2013 Jul 20.
Cronobacter spp. (formerly Enterobacter sakazakii) are emerging, opportunistic pathogens that are linked with food-borne infections in neonates and infants. In the present study, 291 samples of food, 36 samples from a dairy farm and 140 samples of dust from vacuum cleaners were examined for the presence of Cronobacter spp. using chromogenic media and biochemical tests. Altogether, 72 Cronobacter spp. strains were isolated in accordance with the reference standard ČSN P ISO/TS 22964 (2006). No Cronobacter spp. strains were detected in 10 samples of infant milk formula or in samples from a dairy farm. Twelve out of 20 positive food samples were dry products. The incidence of Cronobacter spp. in instant and powdered products and spices (12 positive isolates out of 82 samples) was significantly higher than that in other foods (P = 0.002), but lower than that in samples of dust (52 isolates; P < 0.001). The incidence of Cronobacter spp. in dust from restaurants, bars and hotels (13 positive isolates in 20 samples) was significantly higher than that in dust from households (P = 0.010). The polymerase chain reaction assay for the species-specific detection of the rpoB gene was performed in 49 isolates. Thirty-four Cronobacter spp. isolates were identified as Cronobacter sakazakii, nine isolates as Cronobacter malonaticus and one isolate as Cronobacter turicensis.
阪崎克罗诺杆菌（原阪崎肠杆菌）是一种新兴的机会致病菌，与新生儿和婴儿的食源性感染有关。本研究使用显色培养基和生化试验，对 291 份食品样本、36 份来自奶牛场的样本和 140 份来自吸尘器的灰尘样本进行了阪崎克罗诺杆菌的检测。共按照参考标准ČSN P ISO/TS 22964（2006）分离出 72 株阪崎克罗诺杆菌。10 份婴儿配方奶粉样本和奶牛场样本中均未检测到阪崎克罗诺杆菌。20 份阳性食品样本中有 12 份为干燥产品。速食和粉状产品以及香料（82 份样本中有 12 个阳性分离株）中阪崎克罗诺杆菌的发生率明显高于其他食品（P = 0.002），但低于灰尘样本（52 个分离株；P < 0.001）。餐厅、酒吧和酒店的灰尘（20 个样本中有 13 个阳性分离株）中阪崎克罗诺杆菌的发生率明显高于家庭灰尘（P = 0.010）。对 49 个分离株进行了种特异性 rpoB 基因聚合酶链反应检测。34 株阪崎克罗诺杆菌鉴定为阪崎肠杆菌，9 株鉴定为克罗诺杆菌马罗尼氏菌，1 株鉴定为克罗诺杆菌图里ensis。
