大鼠植入多孔丝素蛋白膜后新生血管形成的分析

Analysis of neovascularization after implanting porous silk fibroin films in rats.

作者信息

Sun Ziling, Bai Lun

机构信息

National Engineering Laboratory for Modern Silk, Suzhou, China.

出版信息

Biomed Mater Eng. 2011;21(4):259-70. doi: 10.3233/BME-2011-0674.

DOI:10.3233/BME-2011-0674

PMID:22182793

Abstract

The purpose of this paper is to explore neovascularization in the biomaterials implanted in vivo. Capillaries formation of dermis defect filled with porous silk fibroin films (PSFFs) implant were investigated in rat at different times. Vascular endothelial growth factor (VEGF), CD34 and hypoxia inducible factor-1 (HIF) were observed in inflammatory cells and fibroblast in PSFFs by day 1, but expression in HIF and VEGF decreased by day 7, otherwise VEGF expressed in vascular endothelial cell increased by day 15 and 25, respectively. VEGF, angiopoietin 1 (Ang-1) and integrins αv and integrins β3 in PSFFs were observed by Reverse transcription-polymerase chain reaction (RT-PCR). Polyvinyl alcohol (PVA) sponge was control. PSFFs have better neovascularization than that of PVA expression of VEGF, HIF and angiogenesis in immunohistochemical and mRNA level.

摘要

本文旨在探究体内植入生物材料中的新生血管形成情况。在大鼠体内，于不同时间点研究了填充有多孔丝素蛋白膜（PSFFs）的真皮缺损处的毛细血管形成情况。在第1天时，在PSFFs中的炎性细胞和成纤维细胞中观察到血管内皮生长因子（VEGF）、CD34和缺氧诱导因子-1（HIF），但到第7天时HIF和VEGF的表达下降，相反，在第15天和第25天时，血管内皮细胞中VEGF的表达分别增加。通过逆转录-聚合酶链反应（RT-PCR）观察了PSFFs中的VEGF、血管生成素1（Ang-1）以及整合素αv和整合素β3。以聚乙烯醇（PVA）海绵作为对照。在免疫组织化学和mRNA水平上，PSFFs在VEGF、HIF表达及血管生成方面比PVA具有更好的新生血管形成能力。