Huang Quan, Yang Jicheng, Miao Jingcheng, Liu Chunyu, Xie Yufeng, Sheng Weihua, Li Mingzhong
Medical School, Soochow University, Suzhou 215123, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2009 Feb;26(1):110-5.
As a biomaterial to be used for reparation in the case of trauma, the silk fibroin, particularly its effect on the transcription and expression of VEGF gene, is a concern. In this study, the ECV304 cell's growth shape and growth curve on the regenerated silk fibroin film were observed, and its VEGF secretion level was measured by ELISA test. It was found that the regenerated silk fibroin film did not interfere with ECV304 cell's growth and function. The L929 cell transfected with human VEGF gene grew on the regenerated silk fibroin film; the real-time quantitative RT-PCR method and ELISA test were used for detecting the transcription and expression of VEGF gene. The results showed the regenerated silk fibroin film did not interfere with the transcription and expression of VEGF gene. Therefore, the regenerated silk fibroin film is a safe biomaterial for inducing vascularization with no untoward effect on the reparation of trauma.
作为一种用于创伤修复的生物材料，丝素蛋白，尤其是其对VEGF基因转录和表达的影响，是人们关注的问题。本研究观察了再生丝素蛋白膜上ECV304细胞的生长形态和生长曲线，并通过ELISA试验测定其VEGF分泌水平。发现再生丝素蛋白膜不干扰ECV304细胞的生长和功能。转染人VEGF基因的L929细胞在再生丝素蛋白膜上生长；采用实时定量RT-PCR方法和ELISA试验检测VEGF基因的转录和表达。结果表明，再生丝素蛋白膜不干扰VEGF基因的转录和表达。因此，再生丝素蛋白膜是一种安全的生物材料，可诱导血管生成，对创伤修复无不良影响。
