Wang Walter, Huang Chih-Yang, Tsai Fuu-Jen, Tsai Chin-Chuan, Yao Chun-Hsu, Chen Yueh-Sheng
School of Chinese Medicine, China Medical University, Taichung, Taiwan.
Int J Artif Organs. 2011 Nov;34(11):1095-105. doi: 10.5301/ijao.5000064.
The present study evaluated in vitro and in vivo the effects of quercetin (QC), a major ingredient in various flavonoids, on peripheral nerve regeneration.
In the in vitro study, we found that QC at concentrations of 0.1, 1, and 10 µg/mL could significantly promote the survival and outgrowth of cultured Schwann cells as compared with the controls treated with culture medium only. In the in vivo study, we evaluated peripheral nerve regeneration across a 15-mm gap in the sciatic nerve of the rat, using a silicone rubber nerve chamber filled with the QC solution. In the control group, the chambers were filled with normal saline only.
At the end of 8 weeks, morphometric data revealed that all 3 QC groups significantly increased the count and density of myelinated axons as compared with the controls. Electrophysiological measurements showed that the QC-treated group at 1 µg/mL had a significantly larger area of evoked muscle action potential (MAP) compared with the controls. In addition, the amplitude of the MAP in the QC-treated groups at 0.1 and 1 µg/mL was significantly larger than that in the controls.
All of these results indicate that QC treatment has nerve growth-promoting effects which may lead to a promising herbal medicine for the recovery of regenerating peripheral nerves.
本研究在体外和体内评估了槲皮素（QC）（多种黄酮类化合物中的主要成分）对周围神经再生的影响。
在体外研究中，我们发现与仅用培养基处理的对照组相比，浓度为0.1、1和10μg/mL的QC可显著促进培养的雪旺细胞的存活和生长。在体内研究中，我们使用填充有QC溶液的硅橡胶神经腔，评估大鼠坐骨神经15毫米间隙处的周围神经再生。在对照组中，神经腔仅填充生理盐水。
在8周结束时，形态学数据显示，与对照组相比，所有3个QC组均显著增加了有髓轴突的数量和密度。电生理测量表明，与对照组相比，1μg/mL的QC处理组诱发肌肉动作电位（MAP）的面积显著更大。此外，0.1和1μg/mL的QC处理组中MAP的幅度显著大于对照组。
所有这些结果表明，QC治疗具有促进神经生长的作用，这可能会产生一种有望用于恢复再生周围神经的草药。
