酵母组蛋白H3/H4突变体文库的构建及筛选方法
Strain construction and screening methods for a yeast histone H3/H4 mutant library.
作者信息
Dai Junbiao, Boeke Jef D
机构信息
School of Life Sciences, Tsinghua University, Beijing, China.
出版信息
Methods Mol Biol. 2012;833:1-14. doi: 10.1007/978-1-61779-477-3_1.
PMID:22183584
Abstract
A mutant library consisting of hundreds of designed point and deletion mutants in the genes encoding Saccharomyces cerevisiae histones H3 and H4 is described. Incorporation of this library into a suitably engineered yeast strain (e.g., bearing a reporter of interest), and the validation of individual library members is described in detail.
摘要
描述了一个由数百个编码酿酒酵母组蛋白H3和H4的基因中的设计点突变和缺失突变体组成的突变体文库。详细介绍了将该文库整合到经过适当工程改造的酵母菌株（例如，带有感兴趣的报告基因）中以及对各个文库成员进行验证的过程。
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