Trivedi Ila, Rai Krishan Mohan, Singh Sunil Kumar, Kumar Verandra, Singh Mala, Ranjan Amol, Lodhi Niraj, Sawant Samir V
National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow, UP, India.
Methods Mol Biol. 2012;833:225-36. doi: 10.1007/978-1-61779-477-3_14.
Histone proteins are the major protein components of chromatin - the physiologically relevant form of the genome (or epigenome) in all eukaryotic cells. For many years, histones were considered passive structural components of eukaryotic chromatin. In recent years, it has been demonstrated that dynamic association of histones and their variants to the genome plays a very important role in gene regulation. Histones are extensively modified during posttranslation viz. acetylation, methylation, phosphorylation, ubiquitylation, etc., and the identification of these covalent marks on canonical and variant histones is crucial for the understanding of their biological significance. Different biochemical techniques have been developed to purify and separate histone proteins; here, we describe techniques for analysis of histones from plant tissues.
组蛋白是染色质的主要蛋白质成分，染色质是所有真核细胞中基因组（或表观基因组）的生理相关形式。多年来，组蛋白一直被认为是真核染色质的被动结构成分。近年来，已证明组蛋白及其变体与基因组的动态关联在基因调控中起着非常重要的作用。组蛋白在翻译后会发生广泛修饰，即乙酰化、甲基化、磷酸化、泛素化等，而在经典组蛋白和变体组蛋白上鉴定这些共价标记对于理解其生物学意义至关重要。已开发出不同的生化技术来纯化和分离组蛋白；在此，我们描述从植物组织中分析组蛋白的技术。
