利用荧光共振能量转移分析核小体构象的变化
Analysis of changes in nucleosome conformation using fluorescence resonance energy transfer.
作者信息
Shahian Tina, Narlikar Geeta J
机构信息
Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA.
出版信息
Methods Mol Biol. 2012;833:337-49. doi: 10.1007/978-1-61779-477-3_20.
PMID:22183603
Abstract
ATP-dependent nucleosome-remodeling motors use the energy of ATP to alter the accessibility of the underlying DNA. Understanding how these motors alter nucleosome structure can be aided by following changes in histone-DNA contacts in real time. Here, we describe a fluorescence resonance energy transfer-based approach that enables visualization of such changes.
摘要
ATP 依赖的核小体重塑马达利用 ATP 的能量来改变其下方 DNA 的可及性。实时跟踪组蛋白与 DNA 的接触变化有助于理解这些马达如何改变核小体结构。在此，我们描述了一种基于荧光共振能量转移的方法，该方法能够可视化此类变化。
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