通过一种与Gateway(®)兼容的二元马铃薯X病毒表达载体从植物病原体中鉴定诱导HR的cDNA。
Identification of HR-inducing cDNAs from plant pathogens via a Gateway(®)-compatible binary Potato virus X-expression vector.
作者信息
van Esse H Peter
机构信息
Laboratory of Phytopathology, Wageningen University, Wageningen, the Netherlands.
出版信息
Methods Mol Biol. 2012;835:97-105. doi: 10.1007/978-1-61779-501-5_6.
PMID:22183649
Abstract
Identification of pathogen effectors that elicit a hypersensitive response (HR) in resistant plant hosts is essential to study disease resistance. In this method, it is described how to generate a cDNA library, how to transfer the library into a binary PVX-expression vector, and finally how to set up a high-throughput screen for HR-inducing cDNAs from plant pathogens.
摘要
鉴定能在抗性植物宿主中引发超敏反应（HR）的病原体效应子对于研究抗病性至关重要。在本方法中，将描述如何构建cDNA文库、如何将文库转移到二元PVX表达载体中，以及最后如何建立一个高通量筛选体系来筛选来自植物病原体的能诱导HR的cDNA。
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