Use of potato virus X (PVX)-based vectors for gene expression and virus-induced gene silencing (VIGS).

Potato virus X-based vectors are a well established system for rapid in planta studies. The vectors can be used for expression of proteins in plants and to down-regulate genes through virus-induced gene silencing. The development of binary-based vectors for Agrobacterium delivery makes this system well suited to high-throughput studies. Protocols are given for establishing infections to achieve expression and VIGS through in vitro transcription and Agrobacterium delivery to glasshouse and in vitro-grown plant material.