真菌基因组中的随机插入诱变以鉴定毒力因子。

Random insertional mutagenesis in fungal genomes to identify virulence factors.

作者信息

Santhanam Parthasarathy

机构信息

Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands.

出版信息

Methods Mol Biol. 2012;835:509-17. doi: 10.1007/978-1-61779-501-5_31.

DOI:10.1007/978-1-61779-501-5_31

PMID:22183674

Abstract

Agrobacterium tumefaciens-mediated transformation (ATMT) has become an important tool for functional genomics in fungi. ATMT-based approaches such as random insertional mutagenesis and targeted knockout are widely used for gene functional analysis in plant-pathogen interactions. Here, we describe a protocol for the identification of pathogenicity and virulence genes through random insertional mutagenesis using the fungal wilt pathogen Verticillium dahliae as an example for the protocol.

摘要

根癌农杆菌介导的转化（ATMT）已成为真菌功能基因组学的重要工具。基于ATMT的方法，如随机插入诱变和靶向敲除，广泛用于植物-病原体相互作用中的基因功能分析。在此，我们以真菌枯萎病病原体大丽轮枝菌为例，描述一种通过随机插入诱变鉴定致病性和毒力基因的方案。