Wang X Q, Feng C H, Lin Z X, Zhang X L
National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, Hubei, China.
Genet Mol Res. 2011 Dec 8;10(4):3620-31. doi: 10.4238/2011.December.8.5.
In order to evaluate the genetic diversity of sea-island cotton (Gossypium barbadense), 237 commonly mapped SSR markers covering the cotton genome were used to genotype 56 sea-island cotton accessions. A total of 218 polymorphic primer pairs (91.98%) amplified 361 loci, with a mean of 1.66 loci. Polymorphism information content values of the SSR primers ranged from 0.035 to 0.862, with a mean of 0.320. The highest mean polymorphism information content value for the SSR motifs was from a compound motif (0.402), and for the chromosomes it was Chr10 (0.589); the highest ratio of polymorphic primers in Xinjiang accessions was from Chr21 (83.33%). Genetic diversity was high in Xinjiang accessions. AMOVA showed that variation was 8 and 92% among populations and within populations, respectively. The 56 sea-island accessions were divided into three groups in the UPGMA dendrogram: Xinhai5 was in the first group; accessions from Xinjiang, except the five main ones, were in the second group, and the other 34 accessions were in the third group. Accessions from the former Soviet Union and Xinjiang main accessions were closely related. Both PCA and UPGMA confirmed that Xinhai5 was distinct from the other accessions, and accessions from Xinjiang were in an independent group. Given the differences between principal components analysis and UPGMA results, it is necessary to combine molecular markers and pedigree information so that genetic diversity can be objectively analyzed.
为了评估海岛棉（Gossypium barbadense）的遗传多样性，使用覆盖棉花基因组的237个常用SSR标记对56份海岛棉种质进行基因分型。总共218对多态性引物（91.98%）扩增出361个位点，平均每个引物扩增出1.66个位点。SSR引物的多态性信息含量值范围为0.035至0.862，平均值为0.320。SSR基序的最高平均多态性信息含量值来自复合基序（0.402），染色体的最高值来自Chr10（0.589）；新疆种质中多态性引物比例最高的是Chr21（83.33%）。新疆种质的遗传多样性较高。方差分析表明，群体间和群体内的变异分别为8%和92%。在UPGMA聚类图中，56份海岛棉种质被分为三组：新海5号在第一组；除五个主要品种外的新疆种质在第二组，其他34份种质在第三组。前苏联种质与新疆主要种质密切相关。主成分分析和UPGMA都证实新海5号与其他种质不同，新疆种质自成一组。鉴于主成分分析和UPGMA结果存在差异，有必要结合分子标记和系谱信息，以便客观地分析遗传多样性。
