亚洲棉种质资源的遗传多样性与亲缘关系分析

Genetic diversity and relationship analysis of Gossypium arboreum accessions.

作者信息

Liu F, Zhou Z L, Wang C Y, Wang Y H, Cai X Y, Wang X X, Zhang Z S, Wang K B

机构信息

College of Agronomy and Biotechnology, Southwest University, Chongqing, China.

State Key Laboratory of Cotton Biology, China/Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang, China.

出版信息

Genet Mol Res. 2015 Nov 19;14(4):14522-9. doi: 10.4238/2015.November.18.15.

DOI:10.4238/2015.November.18.15

PMID:26600511

Abstract

Simple sequence repeat techniques were used to identify the genetic diversity of 101 Gossypium arboreum accessions collected from India, Vietnam, and the southwest of China (Guizhou, Guangxi, and Yunnan provinces). Twenty-six pairs of SSR primers produced a total of 103 polymorphic loci with an average of 3.96 polymorphic loci per primer. The average of the effective number of alleles, Nei's gene diversity, and Shannon's information index were 0.59, 0.2835, and 0.4361, respectively. The diversity varied among different geographic regions. The result of principal component analysis was consistent with that of unweighted pair group method with arithmetic mean clustering analysis. The 101 G. arboreum accessions were clustered into 2 groups.

摘要

采用简单序列重复技术对从印度、越南以及中国西南部（贵州、广西和云南省）收集的101份亚洲棉种质资源的遗传多样性进行了鉴定。26对SSR引物共产生了103个多态性位点，平均每对引物有3.96个多态性位点。有效等位基因数、Nei氏基因多样性和香农信息指数的平均值分别为0.59、0.2835和0.4361。不同地理区域的多样性存在差异。主成分分析结果与算术平均非加权配对组法聚类分析结果一致。101份亚洲棉种质资源被聚类为2组。