Department of Anatomy and Cell Biology, Graduate School of Medicine, Osaka City University, Abenoku, Osaka, Japan.
Dev Dyn. 2012 Feb;241(2):284-93. doi: 10.1002/dvdy.23714. Epub 2011 Dec 19.
The cardiac progenitor cells for the outflow tract (OFT) reside in the visceral mesoderm and mesodermal core of the pharyngeal region, which are defined as the secondary and anterior heart fields (SHF and AHF), respectively.
Using chick embryos, we injected fluorescent-dye into the SHF or AHF at stage 14, and the destinations of the labeled cells were examined at stage 31. Labeled cells from the right SHF were found in the myocardium on the left dorsal side of the OFT, and cells from the left SHF were detected on the right ventral side of the OFT. Labeled cells from the right and left AHF migrated to regions of the ventral wall of the OFT close to the aortic and pulmonary valves, respectively.
These observations indicate that myocardial progenitors from the SHF and AHF contribute to distinct conotruncal regions and that cells from the SHF migrate rotationally while cells from the AHF migrate in a non-rotational manner.
流出道（OFT）的心脏祖细胞位于内脏中胚层和咽区的中胚层核心，分别定义为次级和前心脏区（SHF 和 AHF）。
我们在 14 阶段将荧光染料注射到 SHF 或 AHF 中，然后在 31 阶段检查标记细胞的去向。来自右侧 SHF 的标记细胞被发现在 OFT 的左侧背侧心肌中，而来自左侧 SHF 的细胞被检测到在 OFT 的右侧腹侧。来自右侧和左侧 AHF 的标记细胞迁移到 OFT 腹侧壁靠近主动脉瓣和肺动脉瓣的区域。
这些观察表明，来自 SHF 和 AHF 的心肌祖细胞有助于形成不同的圆锥干区域，来自 SHF 的细胞以旋转方式迁移，而来自 AHF 的细胞以非旋转方式迁移。
