鸡脂肪组织和骨骼肌中 microRNA 的鉴定和特征分析。

Identification and characterization of microRNA from chicken adipose tissue and skeletal muscle.

机构信息

Department of Life Science and Technology, Changshu Institute of Technology, Changshu, 215500, P. R. China.

出版信息

Poult Sci. 2012 Jan;91(1):139-49. doi: 10.3382/ps.2011-01656.

DOI:10.3382/ps.2011-01656

Abstract

MicroRNA (miRNA) is small noncoding RNA that is extensively expressed in organisms. Different types play important roles in various biological processes, such as growth and development. In this study, we identified 47 miRNA in chicken adipose tissue and skeletal muscle, of which 38 were known chicken miRNA, 4 were known miRNA homologous to other species, and 5 were potentially novel miRNA. The target genes from adipose tissue and skeletal muscle were predicted. The expression assay indicated that the 10 selected miRNA were differentially expressed in different developmental stages. Both miRNA-133a and miR-1a were muscle-related, whereas miR-122 was adipose-related miRNA. Certain identified miRNA may be essential to growth and development of chicken adipose tissue and skeletal muscle. Further studies of these miRNA will help to understand their functions in growth and development of adipose tissue and skeletal muscle of poultry.

摘要

微小 RNA（miRNA）是一种广泛表达于生物体中的小型非编码 RNA。不同类型的 miRNA 在各种生物过程中发挥着重要作用，如生长和发育。在本研究中，我们鉴定了鸡脂肪组织和骨骼肌中的 47 种 miRNA，其中 38 种是已知的鸡 miRNA，4 种是与其他物种同源的 miRNA，5 种是潜在的新 miRNA。预测了来自脂肪组织和骨骼肌的靶基因。表达分析表明，10 种选定的 miRNA 在不同发育阶段的表达存在差异。miRNA-133a 和 miR-1a 均与肌肉相关，而 miR-122 是与脂肪相关的 miRNA。某些鉴定出的 miRNA 可能对鸡脂肪组织和骨骼肌的生长和发育至关重要。对这些 miRNA 的进一步研究将有助于了解它们在禽类脂肪组织和骨骼肌生长和发育中的功能。