Fu Shouyi, Zhao Yinli, Li Yuanfang, Li Guoxi, Chen Yi, Li Zhuanjian, Sun Guirong, Li Hong, Kang Xiangtao, Yan Fengbin
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zheng Zhou, Henan Province, P. R. China.
College of Biological Engineering, Henan University of Technology, Zheng Zhou, Henan Province, P. R. China.
J Cell Biochem. 2018 Aug;119(8):7063-7079. doi: 10.1002/jcb.27024. Epub 2018 May 8.
Studies of the miRNA expression profiles associated with the postnatal late development of skeletal muscle and IMF deposition are lacking in chicken. Here, we evaluated the patterns of muscle fiber growth and IMF deposition in breast muscle in the Chinese domestic breed called Gushi chicken, where we constructed four small RNA libraries from breast muscle tissues at 6, 14, 22, and 30 weeks. A total of 388 known miRNAs and 31 novel miRNAs were identified based on four small RNA libraries. Comparative analysis identified 92 significant differentially expressed (SDE) miRNAs based on six combinations. KEGG pathway analysis for the SDE miRNAs showed that metabolic pathways such as glycolysis and biosynthesis of amino acids were significantly enriched before 22 weeks, and pathways such as biosynthesis of unsaturated fatty acids and fatty acid elongation were significantly enriched after 22 weeks. This trend was consistent with the patterns of breast muscle fiber growth and IMF deposition in Gushi chickens. We also constructed miRNA-mRNA interaction networks related to breast muscle development and IMF deposition. The results showed that miRNAs such as gga-miR-1a-3p, and gga-miR-133a-5p may play important roles in breast muscle development, and miRNAs such as gga-miR-103-3p, and gga-miR-138-2-3p may have key roles in IMF deposition. This study determined the dynamic miRNA transcriptome in breast muscle tissue for the first time in Gushi chickens. The results provide a valuable resource for investigating the post-transcriptional regulation mechanisms during postnatal late development of breast muscle and IMF deposition and for evaluating the muscular disease.
关于鸡出生后骨骼肌后期发育和肌内脂肪沉积相关的微小RNA（miRNA）表达谱的研究尚属空白。在此，我们评估了中国地方品种固始鸡胸肌中肌纤维生长和肌内脂肪沉积的模式，我们从6周、14周、22周和30周的胸肌组织构建了四个小RNA文库。基于这四个小RNA文库，共鉴定出388个已知miRNA和31个新miRNA。通过比较分析，基于六种组合鉴定出92个显著差异表达（SDE）的miRNA。对SDE miRNA的KEGG通路分析表明，糖酵解和氨基酸生物合成等代谢通路在22周前显著富集，而不饱和脂肪酸生物合成和脂肪酸延长等通路在22周后显著富集。这一趋势与固始鸡胸肌纤维生长和肌内脂肪沉积的模式一致。我们还构建了与胸肌发育和肌内脂肪沉积相关的miRNA- mRNA相互作用网络。结果表明，gga-miR-1a-3p和gga-miR-133a-5p等miRNA可能在胸肌发育中起重要作用，而gga-miR-103-3p和gga-miR-138-2-3p等miRNA可能在肌内脂肪沉积中起关键作用。本研究首次确定了固始鸡胸肌组织中动态的miRNA转录组。这些结果为研究胸肌出生后后期发育和肌内脂肪沉积过程中的转录后调控机制以及评估肌肉疾病提供了宝贵的资源。
