Matsushita Makoto, Komoda Tsugikazu
Department of Clinical Chemistry, Saitama Prefectural University, Koshigaya 343-8540, Japan.
Rinsho Byori. 2011 Oct;59(10):923-9.
We previously reported that two isoforms of intestinal alkaline phosphatase (IAP) are present in the serum, a high-molecular-weight isoform(HIAP) and a normal-molecular-weight isoform (NIAP), and that both are present at high levels in blood group B or O secretors. In the present paper, we investigated the relationship between effects of high-fat meal and blood groups on ALP activity. Subjects fasted for 14 hours after dinner the previous evening and ate a high-fat meal the following morning. Two types of meals were prepared; a low-calorie meal (470 kcal), and a high-calorie meal (950 kcal). Subjects ate the 2 types of meal on different days. Blood was collected 3 times; once preprandially, and at 3 and 6 h postprandially. Among B or O secretors (n = 24), the mean +/- SD for increase in ALP activity after the high-fat meal was 26.4 +/- 10.2 U/L and 23.3 +/- 9.0 U/L at 3 and 6 h postprandially, respectively, following the low-calorie meal, and 47.9 +/- 19.9 U/L and 55.1 +/- 21.9 U/L at 3 and 6 h postprandially, respectively, after the high-calorie meal. Thus, ALP activity increased 2-fold after the high-calorie meal. Similarly, among subjects with other blood groups (n = 28), the increase in ALP activity was 5.7 +/- 3.7 U/L and 4.2 +/- 3.1 U/L at 3 and 6 h postprandially, respectively, with the low-calorie meal and 8.5 +/- 5.2 U/L and 10.6 +/- 6.0 U/L at 3 and 6 h postprandially, respectively, with the high-calorie meal. Thus, significant differences were seen between the blood groups (p < 0.001). The increases in ALP activity after the high-fat meal were nearly identical to the increases in NIAP activity. These results suggest that a high-fat meal is more likely to affect ALP activity in blood group B or O secretors, and that this effect peaks between 3 and 6 h after the high-fat meal. Taken together, the present results indicate that, as a rule, blood samples for determining ALP activity should be collected in the early morning with the patient in a fasted state.
我们之前报道过，血清中存在两种肠碱性磷酸酶（IAP）同工型，一种是高分子量同工型（HIAP），另一种是正常分子量同工型（NIAP），并且在B型或O型分泌型个体的血液中这两种同工型的含量都很高。在本文中，我们研究了高脂餐的影响与血型对碱性磷酸酶（ALP）活性之间的关系。受试者在前一晚晚餐后禁食14小时，于次日早晨进食高脂餐。准备了两种类型的餐食：低热量餐（470千卡）和高热量餐（950千卡）。受试者在不同日期食用这两种类型的餐食。在餐前、餐后3小时和6小时采集三次血液。在B型或O型分泌型个体（n = 24）中，高脂餐后ALP活性增加的平均值±标准差在餐后3小时和6小时分别为26.4±10.2 U/L和23.3±9.0 U/L（低热量餐后），以及47.9±19.9 U/L和55.1±21.9 U/L（高热量餐后）。因此，高热量餐后ALP活性增加了两倍。同样，在其他血型的受试者（n = 28）中，低热量餐后ALP活性在餐后3小时和6小时分别增加5.7±3.7 U/L和4.2±3.1 U/L，高热量餐后分别增加8.5±5.2 U/L和10.6±6.0 U/L。因此，不同血型之间存在显著差异（p < 0.001）。高脂餐后ALP活性的增加与NIAP活性的增加几乎相同。这些结果表明，高脂餐更有可能影响B型或O型分泌型个体的ALP活性，并且这种影响在高脂餐后3至6小时达到峰值。综上所述，目前的结果表明，通常情况下，测定ALP活性的血样应在清晨患者空腹状态下采集。
